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Human CD3E/G HEK293 Cell Lysate

Human CD3E/G HEK293 Cell Lysate

Cat.No. :  CLOE-0011 Host Cell: HEK293

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Cell Lysate Information

Cat. No. CLOE-0011
Description This cell lysate over-expresses the extracellular domains of human CD3E and CD3G. Human CD3E protein fragment is fused with polyhistidine and the Fc region of human IgG1 at the C-terminus. Human CD3G protein fragment is fused with the Fc region of mouse IgG1 at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene CD3E/G
Host Cell HEK293
Species Homo sapiens (Human)
Applications Positive control for Western Blot (WB), antibody verification, protein purification
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Shipping Ice package
Storage Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
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