Human CD3D/E HEK293 Cell Lysate

Name: Human CD3D/E HEK293 Cell Lysate
SKU: CLOE-0013
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0013 Host Cell : HEK293

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Cell Lysate Information

Cat. No.	CLOE-0013
Description	This cell lysate over-expresses the extracellular domains of human CD3D and CD3E. Human CD3D protein fragment is fused with FLAG at the C-terminus. Human CD3E protein fragment is fused with polyhistidine at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses the extracellular domains of human CD3D and CD3E. Human CD3D protein fragment is fused with FLAG at the C-terminus. Human CD3E protein fragment is fused with polyhistidine at the C-terminus. Purification of target protein
Target Gene	CD3D/E
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

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