DNA-näytteiden puhdistuksen automatisointi Biomek-robotilla

Authors: Raimi, Viktor;
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Publisher: Metropolia Ammattikorkeakoulu

Abstract

This Bachelor's thesis studies the automation of the polyethylene glycol purification protocol with the Beckman Biomek NXp pipetting robot as well as discusses the steps of automation and the user-friendliness of the security aspects. The PEG purification used for DNA purification is a tedious and slow step by hand, which is why the protocol needs to be automated using a pipetting robot. The thesis project also validates the superparamagnetic carboxyl beads of different manufacturers. The beads play a central role in PEG purification by binding DNA fragments. After validation of the programming and bead validation, compatibility validation was performed. The aim was to verify the suitability of the beads for the automated system. During the thesis project, an unexplained disappearance of the DNA sample occurred, which eventually turned out to be a residual reagent from the process making it seem as if the sample was disappearing.