Directed editing of cellular rna via nuclear delivery of crispr/cas9

Authors: Yeo, Eugene; Brannan, Kristopher; Marina, Ryan; Nelles, David;
Patent

Publisher: Google Patents

Abstract

Disclosed herein is a technology to perform programmable RNA editing at single-nucleotide resolution using RNA-targeting CRISPR/Cas9. This approach, which Applicants have termed "Cas9-directed RNA editing" or "CREDIT," provides a means to reversibly alter genetic information in a temporal manner, unlike traditional CRISPR/Cas9 driven genomic engineering which relies on permanently altering DNA sequence.