Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-21
| Cat. No. | CC-21 |
| Description | All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with Monocots, especially with rice. The Monocots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9. |
| Efficiency | SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency. |
| Features |
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| Applications | The Monocots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots. |
| Resistance | Kanamycin |
| Selection Marker | Hygromycin B |
A: The expression of Hygromycin phosphotransferase (Hpt) is usually driven by a strong constitutive promoter, such as maize Ubiquitin 1 (ZmUbi1), rice ACTIN 1, or Cauliflower mosaic virus (CaMV) 35S, for the ubiquitous expression of the antibiotic-tolerance gene.
A: In the process of application, the modification of Cas9 protein and the selection of promoters will affect the efficiency of genome editing in monocots.
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Great kit. Using Monocots SaCas9 Genome Editing Kit, I have successfully induced specific base conversions in the rice genome.
The CRISPR/SaCas9 system will be more accurate in creating mutations, because SaCas9 recognizes a longer PAM motif than SpCas9.
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