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Monocots SaCas9 Genome Editing Kit (Hygromycin)

For research use only. Not intended for any clinical use.
Cat.No.
CC-21
Description
All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with Monocots, especially with rice. The Monocots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9.
Applications
The Monocots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots.
Efficiency
SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of SaCas9 protein in dicots by using ubiquitin promoter and dicot codon optimized-SaCas9.
•High activity of sgRNA in Arabidopsis by using OsU6 (rice U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Hygromycin B

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Q & A

Customer Reviews

Customer Q&As
Which promoter drives the expression of Hygromycin?

A: The expression of Hygromycin phosphotransferase (Hpt) is usually driven by a strong constitutive promoter, such as maize Ubiquitin 1 (ZmUbi1), rice ACTIN 1, or Cauliflower mosaic virus (CaMV) 35S, for the ubiquitous expression of the antibiotic-tolerance gene.

What do factors affect the efficiency of monocots genome editing?

A: In the process of application, the modification of Cas9 protein and the selection of promoters will affect the efficiency of genome editing in monocots.

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Customer Reviews
Fast & Convenient

Great kit. Using Monocots SaCas9 Genome Editing Kit, I have successfully induced specific base conversions in the rice genome.

United States

12/02/2021

Worked Very Well

The CRISPR/SaCas9 system will be more accurate in creating mutations, because SaCas9 recognizes a longer PAM motif than SpCas9.

United States

02/06/2022

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