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AANAT Easy KO Kit

For research use only. Not intended for any clinical use.
Cat.No.
CC-07
Description
A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Applications
This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Species
Human

Publications

Q & A

Customer Reviews

Customer Q&As
Why did my CRISPR plant construct did not produce the expected target cleavage?

A: Not every sgRNA will produce the target cleavage. It is recommended to use two to three sgRNAs per gene to increase the successful rate.

What antibiotics should I use for selection of the CRISPR rice vectors in Agrobacterium Plantarum?

A: Kanamycin.

What is the promoter used for the expression of Cas9?

A: A codon optimized Cas9 expression cassette is driven by ubiquitin promoter.

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Customer Reviews
Worked Very Well

This kit makes the system much simpler than other engineering nuclease systems. We have successfully knocked out a variety of rice genes, such as plant ene desaturase gene (OsPDS), betaine aldehyde dehydrogenase (OsBADH2), and mitogen-activated protein kinase (OsMPK2).

United States

12/17/2022

Time saving

Time saving. We soon completed the gene editing of rice.

United States

12/18/2022

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