Monocots SaCas9 Genome Editing Kit (Basta)

Name: Monocots SaCas9 Genome Editing Kit (Basta)
SKU: CC-22
Rating: 4.0 (1 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

CC-22

Description

All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with Monocots, especially with rice. The Monocots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9.

Applications

The Monocots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots.

Efficiency

SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.

Features

•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of SaCas9 protein in dicots by using ubiquitin promoter and dicot codon optimized-SaCas9.
•High activity of sgRNA in Arabidopsis by using OsU6 (rice U6) promoter.
•Mutiplex genome editing

Resistance

Kanamycin

Selection Marker

Basta

Publications

Q & A

Customer Reviews

Customer Q&As

What is Basta?

A: Glufosinate-ammonium (PPT/Basta) is a structural analogue of glutamic acid, which competitively inhibits the activity of glutamine synthetase (GS) and interferes with the synthesis of amino acids and glutamine. It is the shortage of glutamine that leads to the inability of the body to detoxify ammonia, the inhibition of photosynthesis, and ultimately the death of plants. Basta is often used as a selective marker in plant genetic engineering to screen transgenic crops resistant to glyphosate.

What is the difference between prime editing and CRISPR?

A: Prime editing, borrowed from CRISPR, is another precise genome editing method that can generate all 12 types of base substitutions, targeted small insertions, deletions, and combinations of these editing results in the target site. The prime editor mainly consists of a catalytically impaired Cas9 (Cas9n, H840A) fused with an engineered reverse transcriptase and a prime editing guide RNA (pegRNA).

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Customer Reviews

Time saving

The kit helps me simplify the process, shorten the production cycle and reduce the cost.

United States

09/10/2022

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Monocots SaCas9 Genome Editing Kit (Basta)

Publications

Q & A

Customer Reviews

What is Basta?

What is the difference between prime editing and CRISPR?

Time saving

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