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The development of the CRISPR/Cas9 genome editing technology has been one of the most exciting events in the field of genome editing. Because of its relative ease of use, researchers have begun to use CRISPR/Cas9 for a wide range of applications, including gene markers, knockout of target genes, and introduction of specific mutations, insertions or deletions. It has been used to create disease models, study gene function, improve agriculture, etc., and is expected to be further applied in the future.
Cas9 is part of the CRISPR genome editing system and used to perform site-specific cleavage of the double-stranded DNA with the guidance of gRNA. CRISPR/Cas9 PlatformCB, a biotechnology company specializing in gene editing research, is committed to providing clients with the most comprehensive and professional CRISPR/Cas9 gene editing services and products. Based on our platform, we are able to provide you with CRISPR-related proteins including a variety of purified spCas9 proteins.
Why choose CRISPR/Cas9 PlatformCB Cas9 Protein?
The Cas9 nuclease of S. pyogenes, abbreviated as spCas9, is the most commonly used variant of Cas9. There are two reasons why spCas9 is popular. First, the spCas9 PAM sequence is 5'-NGG, which is highly abundant in the genome and can target almost any gene. The spCas9 enzyme is more efficient in vivo than other variants.
In addition, we also provide you with Cas9 mutant proteins, which can be used for CRISPR-mediated ChIP, epigenetic modification, live cell gene composition, transcriptional activation/inhibition, etc.