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We are currently undergoing a biotechnology revolution. Genomics advances led by CRISPR/Cas9 technology have greatly accelerated genomic engineering research. As CRISPR opens the door to more and more applications every day, more and more researchers are using this technology for research.
How does the CRISPR/Cas9 system work?
The CRISPR/Cas9 system relies on two main components: guide RNA (gRNA) and CRISPR-related (Cas) nucleases.
gRNA is a specific RNA sequence that recognizes a target region of a target DNA and directs the Cas nuclease to edit there.
gRNA consists of two parts:
➢ CRISPR RNA (crRNA) - A 17-20 nt sequence complementary to the target DNA, a customizable component that achieves specificity in each CRISPR.
➢ Trans-activating crRNA (tracrRNA) - Serving as a binding scaffold for Cas nuclease.
➢ sgRNA (single guide RNA) - As the name suggests, sgRNA is a single RNA molecule that contains a custom-designed short crRNA sequence and a scaffold tracrRNA sequence. sgRNA can be artificially synthesized or prepared from DNA templates in vitro or in vivo.
CRISPR-related protein is a non-specific endonuclease. It is directed by gRNA to a specific DNA locus and forms a double-strand break in it. There are several versions of Cas9 nuclease isolated from different bacteria. The most commonly used is Cas9 nuclease from Streptococcus pyogenes.
What do we offer?
sgRNA design and synthesis
➢ For gene/genome editing, including knockout, knock-in, point mutation
➢ For transcriptional modulation (CRISPR activation or CRISPR interference)
➢ For epigenetic editing (histone acetylation/ demethylation, cytosine methylation/demethylation, etc.)
➢ For enChIP, live imaging of the cellular genome, etc.
Donor DNA design and synthesis
➢ We offer a variety of sgRNA vector designs including sgRNA only, sgRNA-Cas9, dual sgRNA, inducible sgRNA vectors, etc.
➢ We offer Cas9 and dCas9 hybrids (e.g. dCas9-KRAB, dCas9-VPH, dCas9-VPR, etc.)
➢ We offer TET-inducible expression for CRISPR KO, CRISPRa or CRISPRi.
CRISPR off-target effects analysis
Base editing by CRISPR
CRISPR/Cas9 PlasformCB has extensive experience in gene/genome editing. We can provide you with multiple solutions covering the entire process of using the CRISPR system, high-quality end-to-end services, and marketable products, including single sgRNA/sgRNA library design/synthesis, CRISPR vector construction, bioinformatics analysis, etc. Relying on a proprietary gene synthesis platform, we successfully completed the efficient and high-throughput gene/genome editing. There is no doubt CRISPR/Cas9 PlasformCB will be the best partner during your research.