Custom Rat Model    

It has previously been difficult to genetically engineer rats, but these limitations have been overcome by using CRISPR technology. CRISPR/Cas9 Platform^CB is dedicated to creating custom genetically edited rat models to meet specific requirements, including neuroscience, ADMET, cardiovascular and oncology, etc. Compared to traditional methods, our advanced CRISPR/Cas9 service flow enables consistent and precise targeting, eliminating the concerns and additional breeding caused by mosaicism and enabling germline transmission.

Why use a rat model?

For more than 150 years, laboratory mice have been a model for human disease research.

In a wide range of scientific fields, including neurobiology, cardiology, immunology, and toxicology, rats have been used to mimic human disease for more than a century. Their size and physiological similarity to humans make them ideal for many laboratories.

• Mouse models are not always reliable in preclinical studies and have some limitations in mimicking human disease.
• Their metabolism, physiology, and pathology are closer to humans than mice. Rats exhibit a more complex behavior than mice, and larger brains allow for more complex electrophysiological recordings.
• Excellent cognitive and memory, neurological and psychological analysis, and behavioral models for drug screening.
• Superior model of cardiovascular disease (stroke and hypertension), autoimmune diseases, diabetes, diabetes, and autoimmune diseases.
• Complex surgical procedures can be performed on rats, which is difficult or impossible for smaller rodents.

Our customized rat models include

• Constitutive knockout model
• Induced expression of rat
• Point mutation rat model
• Conditional knockout rat model
• Rosa26 knockin model
• fluorescent proteins or immune-tags insertion

Our CRISPR/Cas9 rat model services include

• Strategy design
• gRNA target sites selection and gRNA synthesis.
• Donor DNA design and synthesis (if needed).
• CRISPR vector construction.
• Plasmid microinjection by our skilled injection specialist (we will inject as many embryos as needed to fulfill the guarantee).
• Screening, identification (via PCR and sequencing).
• Deliver the final rat model and final report describing the details of the genome editing and showing evidence of the required editing.

Applications of CRISPR/Cas9 rat model

• Study the function of genes/proteins
• Human disease model
• Drug screening and discovery
• Preclinical safety and toxicity studies

CRISPR/Cas9 Platform^CB is a leading biotech company specializing in offering a set of services for the application of the CRISPR/Cas9 system. Our high-quality service process and efficiency of the experimental cycle meet researchers' demands from the early targeted site selection and sgRNA design to the complete CRISPR/Cas9 vectors and CRISPR/Cas9 edited animals and cell lines, which have acquired the customer's consistent good comments. There is no doubt CRISPR/Cas9 Platform^CB is the best partner during your project.