SpCas9 Nuclease (Dual NLS)
Cat.No.
CCP-003
Description
Recombinant Cas9 protein fused with both N-terminal and C-terminal NLS sequences to maximize nuclear localization.
Activity Assay
Validated endonuclease activity
Applications
Gene editing in mammalian cells requiring maximal nuclear import efficiency
Buffer Composition
20 mM HEPES (pH 7.5), 150 mM KCl, 1 mM DTT, 10% glycerol
Concentration
~10 mg/mL
Endotoxin Level
<5 EU/mg
Expression System
E. coli
Molecular Weight
~164 kDa
Purity
≥95%
Shipping
Dry ice
Source Species
S. pyogenes
Storage
–80 °C
Synonyms
SpCas9-2NLS
For research use only. Not intended for any clinical use.
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