Custom CRISPRa sgRNA Vectors    

CRISPR activation (CRISPRa) is a CRISPR tool that provides a powerful way to activate genes on a large scale in a focused manner. CRISPRa takes full advantage of a modified version of dCas9, a Cas9 mutation that does not have endonuclease activity, and fused with transcriptional activators. The screening of CRISPR-based transcriptional activation has been used to research mechanisms of human diseases, screen drug targets, and establish causal relationships between gene expression and cell fate determination. In addition, CRISPR-based gene activation is expected to be used to target endogenous gene regulatory elements to increase the expression level of normal gene copies to correct diseases caused by haploid function.

For this application, CRISPR/Cas9 Platform^CB provides customized CRISPRa sgRNA vectors construction services, including sgRNA design and viral vector packaging, which can be applied to both easy-to-transfect cells and hard-to-transfect cells. Just send us the gene ID you are interested in, we will design, clone and provide this powerful technology for you. Our custom CRISPRa sgRNA can be purchased separately or as a pre-packaged virus.

Project strategy

Suhyung Cho. 2018

Project workflow

• Consult our technicians and design your CRISPR-based gene activation protocol
• Screening target sites and designing sgRNA sequences - at least three different sgRNA sequences to further improve target gene activation performance.
• Construction of CRISPRa sgRNA vector - Providing different vector designs according to your needs, including sgRNA only, sgRNA-dCas9.

➢ Delivery vectors and project report - Unique technology platform guarantees shorter turnaround time and lower price.

Alternative vectors

• Lentivirus
• Adenovirus
• Adeno-associated virus

Vector details

Service	Mechanisms	Florescent tag	Resistance gene
Custom CRISPRa sgRNA vector	dCas9-VPR system	Available	Available

Vectors carry fluorescent tags (GFP/mCherry, etc.), which can be used to detect the expression, and resistance gene (puromycin/neomycin) used to screen stable expression cells.

Applications

• Strong transcriptional activation in multiple cell lines
• Functional genomics/target verification
• Unbiased positive/negative gene screening - Includes cell-based survival/proliferation, sensitivity to drugs or toxins, screening of fluorescent reporter genes, and single-cell transcriptome.
• Drug discovery and screening targets
• Immunotherapy research

As one of the leading biotechnology companies, CRISPR/Cas9 Platform^CB has experienced experts in gene editing. We provide custom CRISPR activation sgRNA vectors construction services to meet your special needs. We are committed to providing customers with efficient solution and high-quality service to meet your specific needs. If you have any questions, please feel free to contact us. We look forward to working with you in the future.