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CRISPR/Cas9 technology has been used as a genome editing tool for a variety of cell lines, including a variety of tumor cells in humans and mice. In addition, based on its easy operation and high accuracy, CRISPR/Cas9 technology have great potential for application in vitro disease models and disease treatment：
CRISPR/Cas9 PlatformCB, the global leading biotechnology company focused on gene editing, provides a custom gene edited cell line service based on our self-successful CRISPR/Cas9 cell line generation platform. As one of the earliest CRISPR service providers, so far we have designed >1300 unique cell line models in >500 different mammalian cell lines. We can generate a CRISPR cell line model based on your specifications, which takes only 2-3 months.
Different CRISPR-mediated modifications we offered
The workflow of custom CRISPR/Cas9 cell lines generation
Depending on the type of cell required, we prepare the cells two weeks in advance to maintain their high activity.
Based on the nature of the gene, candidate knockout sites are selected and then identified.
Three to five (3-5) sets of gRNA will be designed and construct the corresponding plasmids, while Cas9 is integrated.
The constructed plasmid is transfected into a host cell to produce a cell bank. We will then sequence the cells in the cell bank to verify the knockout effect.
Transfected cells are seeded in 96-well plates for monoclonal screening using limiting dilution methods. Positive monoclonal cells will be amplified, sequenced, identified and stored. Finally, a cell line is successfully constructed.
Mycoplasma detection and quantitative polymerase chain reaction and Western blotting.
Expected turnaround time
CRISPR/Cas9 PlatformCB is specializing in building efficient systems and procedures to meet the needs and timelines of clients working in the CRISPR/Cas9 gene editing. Our scientists have extensive experience with different cells, including easy-to-transfer cell lines and difficult-to-transfect cell lines. Tell us your project needs, we will offer you a one-stop-shop CRISPR/Cas9 gene editing service from strategy design to final stable cell line. We guarantee our clients the most reliable and efficient research services to best match your research goals, and faster turnaround time, lower price. If you have any questions, don’t hesitate to contact us.