sgRNA Bioinformatics Service    

The development of next-generation sequencing technology has greatly promoted the development of precision medicine and personalized medicine. By sequencing, researchers can compare mutations in a gene more directly between different cells. However, there are often many other mixed characteristics that mask the direct relationship between genotype and disease.

With the maturity and universal application of the CRISPR/Cas9 system, researchers will be able to edit target genes in living cells and accurately change the DNA sequence, laying a foundation for developing new therapies for human diseases.

CRISPR/Cas9 Platform^CB can provide professional sgRNA bioinformatics data analysis services according to the specific needs of customers, covering sgRNA design, synthesis, and gene editing. By combining PREEGOC, TEGA, COSMIC and other professional databases, we can analyze and screen gene expression information, gene mutation frequency information, and post-clinical correlation data, and conduct integrated biological genomics analysis to provide potential drug targets and personalized medicine solution. At the same time, it can also help researchers find other additional genes that can be used as therapeutic targets.

Project workflow

• Step 1. Chip synthesis

➢ gRNA design
➢ High throughput gene synthesis

• Step 2. Library construction

➢ Vectors construction
➢ NGS validation

• Step 3. Cell transfection

➢ Virus packaging
➢ Stable plant construction

• Step 4. High-throughput screening

➢ Positive control test - Drug resistance test
➢ Negative controlled trials - Reporter testing

• Step 5. Gene functional analysis and prediction

➢ Genetic structure analysis
➢ Unigene annotation
➢ Functional annotation and enrichment analysis of differential genes

Our characteristics and advantages

• Suitable for many species (human, mouse, rat, zebrafish, nematode, fruit fly, rabbit, etc.)
• With extensive experience in CRISPR/Cas9 sgRNA design, we provide customized services for specific needs
• Professional bioinformatics team provides scientific solution design and in-depth data mining
• Reliable and flexible data reporting
• Unique technology platform ensures fast turnaround times

Application areas

• Basic biological research
• Human disease research
• Drug discovery
• Microbiology
• Agriculture
• Environmental science
• Oceanography, etc.

Next-generation sequencing in gene editing

CRISPR/Cas9 gene editing	Next-generation sequencing enablement
Select gRNA target sites	Genome/gene/loci resequencing
Design gRNA
CRISPR system delivery	Plasmid resequencing
Cell transfection/microinjection
Verification	High resolution on- and off-target assessment
Functional confirmation	RNA-Seq, single-cell RNA-Seq, Chip-Seq, etc.

Our work is based on our knowledge and understanding of the CRISPR system and its applications. More importantly: we are experts in CRISPR gene-editing technology. We guarantee or clients high-quality services and products. If you have any questions or requirements, don't hesitate to contact us.