Microbial Genome Editing Service

Microbial Genome Editing Service    

CRISPR/Cas9 PlatformCB delivers high-quality CRISPR/Cas9-mediated genome editing service for our clients. Our microbial genome editing service are backed by extensive experience, top researchers and biosecure facilities. CRISPR/Cas9 PlatformCB deploys a powerful and flexible gene editing platform to help you every step of the way with innovative bioanalytical solutions in your genome editing workflow.

Homologous recombination has been broadly applied in genome editing of prokaryotes with high efficiency and accuracy. However, this way is limited in realizing larger-scale genome editing with large DNA fragments or numerous genes because DNA editing template construction is a relatively complicated procedure1. Derived from the immune system present in bacteria and archaea, CRISPR technology as an efficient genome-scale editing tool has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering2. Consequently, the CRISPR/Cas9 system has rapidly become a transformative and powerful tool for editing genomes in many organisms.

Fig.1 A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome (Su, et al. 2016)

As a premier partner in microbial genome editing, CRISPR/Cas9 PlatformCB offers a "one-stop shop" tailored to your gene research needs. Our talented scientists have extensive experience in gene knockout, knock-in/replacement and site-directed mutagenesis. CRISPR/Cas9 PlatformCB offers professional and comprehensive services ranging from sgRNA vector construction to genome editing, clonal selection and validation. Our aim is to offer customizable and flexible services and to fit how you run projects.

Workflow of CRISPR/Cas9-mediated genome editing services

Fig.2 Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli (Zerbini, et al. 2017)

Advantages of CRISPR/Cas9 Technology

  • Seamless
  • High efficiency & precision
  • Multigene editing: can knock-out up to 5 genes at the same time
  • Easy selection: no selectable marker is required

As a premier institution in microbial genome editing, CRISPR/Cas9 PlatformCB has years of experience to meet your specific project needs in using the CRISPR/Cas 9 technology to add value to your research project. CRISPR/Cas9 PlatformCB offer a series of services for genetic modification which has been successfully achieved in microorganisms, such as E. coli, Salmonella, Streptomyces, Clostridium, Bacillus subtilis, cyanobacteria, Saccharomyces cerevisiae, filamentous fungi, and so on. Consequently, simply let us know how you want the genome to be edited. We will propose the best strategy for you.

References:

  1. Su, T., Liu, F., Gu, P., Jin, H., Chang, Y., & Wang, Q., et al. (2016). A crispr-cas9 assisted non-homologous end-joining strategy for one-step engineering of bacterial genome. Scientific Reports, 6, 37895.
  2. Tian, P., Wang, J., Shen, X., Rey, J. F., Yuan, Q., & Yan, Y. (2017). Fundamental crispr-cas9 tools and current applications in microbial systems. Synthetic & Systems Biotechnology, 2(3), 219-225.
  3. Zerbini, F., Zanella, I., Fraccascia, D., König, E., Irene, C., & Frattini, L. F., et al. (2017). Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli. Microbial Cell Factories, 16(1), 68.

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