Knockin Cell Line Generation    

CRISPR/Cas9 Platform^CB provides one stop custom knock-in cell line generation service, including point mutation and gene insertion. With non-stop exploration of technology, we have developed an excellent platform for gene editing. Based on the platform, our expert staff has succeeded in dozens of knock-in cell line generation projects, including stem cells, tumor cells and even difficult-to-handle cells. At CRISPR/Cas9 Platform^CB, a full cell line generation service is offered from sgRNA design/construction to final cell line generation/verification.

CRISPR system is a novel tool for precise gene editing. Currently, the most commonly used system is derived from Streptococcus pyogenes (Sp), which consists of a Cas9 nuclease and a guide RNA. SpCas9 will induce a DNA double-stranded break after recruited to specific site by sgRNA. The subsequent homologous recombination repair will generate a knock-in or point mutation phenotype in the presence of a recombination donor. Thus, the HDR pathway are exploited to facilitate correction of diseased genes, insertion of epitope tags or fluorescent reporters, and overexpression of genes of interest in a site-specific manner.

Fig1. CRISPR/Cas9 Knock-in service

CRISPR/Cas9 Platform^CB offers custom knock-in cell line generation service, including:

• Point Mutation Cell Line Generation
• Reporter Genes Knock-in Cell Line Generation
• Custom Gene Knock-in Cell Line Generation

For more information, please feel free to contact us.