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CRISPR/Cas9 Gene Fusion Cell Line Services 
Chromosomal rearrangements (translocations, inversions, deletions or insertions) of independent (isolated) genes result in chimeric genes or gene fusions and have been implicated in many types of cancers (solid tumors and hematological malignancies). Originally identified in the 1980s, these fusion genes have been identified as playing a major role in precision/personal medicine and are used as therapeutic biomarkers for diagnosis, prognosis, and drug development. Since fusion genes are used for diagnostic and prognostic purposes and are targets for treatment, assessing their function in cancer is critical. However, the functional role of these mutations is unclear. Most studies that elucidate the mechanisms of cancer mutations rely on gene knockout/function loss or insertion and overexpression of recombinant genes. However, these cell line models do not reflect the clinical situation of chromosomal translocations.
CRISPR/Cas9 platformCB, one of the leading genetic editing services companies, is specializing in building efficient systems and procedures to meet the needs and timelines of clients working in the CRISPR/Cas9 gene editing. To systematically study the role of fusion genes in tumor cells, we can customize the chromosomal rearrangements (translocation/ reverse/missing/inserting) required for engineering in your chosen cell line.
Workflow of CRISPR/Cas9 gene fusion cell lines services
We provide custom cell lines according to your requirements, our scientists have extensive experience with >200 distinct cell lines and are very familiar with their culture conditions and genetic backgrounds.
Successfully modified mammalian cell lines by CRISPR/Cas9 platformCB: Blood Lineage Cells (RAW264.7, HMC1.2, K562, U937, etc.), Cancer Cell Lines (HEK293, HEK293T, Hela, MCF7, Neuro2a, HepG2, U87, etc.), Stem Cells (iPSC/ ESC), Other Cell Lines (NIH3T3, MCF10, HEME, SW10, etc.).
Our CRISPR/Cas9 gene fusion cell lines services include, but are not limited to:
- Available types of chimeric genes: chromosome level and chimeric cDNA knockin
- Footprint-free genome editing
- Isogenic cell lines are used for reliable comparison of results and elucidate the full potential of mutations
- Multiple analysis of DNA, RNA and protein levels
- RT-PCR, RNA-Seq, immunocytochemistry, fluorescence in situ hybridization and Western blotting
Why choose CRISPR/Cas9 PlatformCB?
- Novel CRISPR strategy for higher efficiency and success rate (>98%)
- Multiple cell lines
- Rich experience: More than thousands of genetic editing project experience
- Custom deliverables: homozygous/hybrid clones
- Shorter turnaround time, lower price
- Milestone update/report and final report
Applications
- Modeling of clinically relevant mutations
- Molecular biomarkers for cancer prognosis research
- Drug discovery and screening goals
- Immunotherapy research
As a professional gene editing services company, CRISPR/Cas9 PlatformCB has successfully obtained thousands of CRISPR/Cas9 cell lines in >200 different cell models, including easy-to-transfer cell lines and difficult-to-transfect cell lines. Tell us your project needs, we will offer you a comprehensive custom CRISPR/Cas9 gene editing service from strategy design to final stable cell line. We guarantee our clients the most reliable and efficient research services to best match your research goals, and faster turnaround time, lower price. If you have any project needing CRISPR/Cas9 cell lines, please feel free to contact us.