Custom Zebrafish Model

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Custom Zebrafish Model    

CRISPR/Cas9 PlatformCB uses the latest CRISPR/Cas9 technology to work with customers to create unique transgenic zebrafish models to meet their research needs. Our expert's team of zebrafish research provides expertise in transgenic manipulation and zebrafish biology for the generation of mutations and/or knock-in lines, and subsequent phenotypic analysis for custom drug screening. Our scientists provide personalized help to help them create and develop specific disease and reporting models.

Our custom zebrafish models include

➢ Gene knockout
➢ Conditional knockout/knockin
➢ Point mutation
➢ Floxed allele insertion
➢ Fluorescent proteins insertion (e.g. GFP, RFP, mCherry, etc.) or immune-tags insertion (e.g. FLAG, HA, etc.)

Workflow of our custom zebrafish model service

Custom Zebrafish Modell

Applications of CRISPR/Cas9 zebrafish

➢ Functional genomics research
➢ Disease research
➢ Toxicity research and drug screening

Why use zebrafish?

Zebrafish is a powerful and versatile model organism that is ideal for basic and applied biomedical researchers.

  • Zebrafish as a development model

The combination of gene editing techniques with fast, externally developed and transparent zebrafish embryos makes zebrafish an important model system for studying vertebrate development. Zebrafish has been very successful in neurodevelopmental research models.

  • Zebrafish as a regeneration model

In addition to its rapid development, zebrafish have a powerful ability to regenerate or replace many cells and tissues that mammals cannot repair, including appendages, heart, brain, and spinal cord, as well as sensory cells in the eyes and ears. However, the use of conditional knockout model technology (inducible expression of Cre or Cas9 enzyme) in zebrafish enables time-specific control of gene expression in target cells, allowing more specific localization of genes in regeneration features.

  • Zebrafish as a behavioral model

Both larvae and adult zebrafish have many behaviors worth studying. Among the well-studied behaviors, including startle response and visual response. Zebrafish are also used to study other more complex behaviors such as sleep/awakening, learning and memory, exercise, and emotional behaviors like depression and the effects of antidepressants.

  • Zebrafish as a disease model

Zebrafish is a powerful model of many different types of cancer in the laboratory. With the further development of the ability to use genome editing techniques, the zebrafish model will be able to study more diseases.

  • Zebrafish as a toxicology model

Some early studies of zebrafish include the use of them to screen for potential carcinogens or teratogenic effects. Large-scale pharmacological screening of zebrafish disease models can be performed by simply placing test compounds in water, which provides a powerful platform for therapeutic discovery.

CRISPR/Cas9 PlatformCB is committed to building effective systems and procedures to meet the needs and timelines of customers working in the development and application of genetic editing technologies. CRISPR/Cas9 PlatformCB provides customized zebrafish model building services, including expert research design, in vitro sgRNA design synthesis, CRISPR vector construction and final zebrafish, allowing you to precisely control the expression of target genes in zebrafish. Our CRISPR/Cas9 zebrafish model service will help advance your developmental and reproductive research, disease research, preclinical drug discovery and toxicology programs, and enhance your organization's ability to make informed decisions about important milestones.

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