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In the CRISPR system, the guide RNA (gRNA) sequence targets the target DNA site and the Cas9 protein is used to perform double-stranded DNA cleavage. When using the CRISPR vector system, the Cas9 mRNA can be transfected at any time and avoid the need for time-consuming cloning steps. Cas9 mRNA is co-transfected with a target-specific gRNA that directs the Cas9 protein to the certain genomic locus to generate a double-strand break. The intact mRNA format has a smaller payload than the plasmid-based Cas9 system, thereby improving cell delivery and improving genome editing efficiency. In addition, Cas9 mRNA can be used in multiple pathways with more than one gRNA. Use this method to determine which gRNA sequence is best for a particular target, or to edit multiple genomic loci by one transfection.
With many years of experience in gene editing, CRISPR/Cas9 PlatformCB is always dedicated to providing a comprehensive range of CRISPR/Cas9 products and services for academic research, biotechnology research, and drug discovery. In order to meet the needs of our clients, we provide high performance Cas9 mRNA sequence with two nuclear localization sequences (NLSs), which has been shown to be more efficient at targeting the Cas9 protein to the nucleus. By using new manufacturing processes, we are able to achieve superior product quality and improved product performance.
Why choose Cas9 mRNA?
In addition, we also provide you with Cas9 mutant mRNA, which can be used for CRISPR-mediated ChIP, epigenetic modification, live cell gene composition, transcriptional activation/inhibition, etc.
If you want to know more about our CRISPR products and services, please feel free to contact us.