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Creative Biogene CRISPR/Cas9 Platform brings a vast range of CRISPR-Cas9 services and products to clients. With years of experience and steady platform, our scientist team is expert in applications of CRISPR-Cas9 system. We offer various CRISPR-Cas9 related services and products to assist you for genome editing at various stages. We are dedicated to providing the most comprehensive, affordable and high-quality CRISPR-Cas9 related services and products to win your satisfaction in a professional manner.

CRISPR/Cas9 PlatformCB offers complete CRISPR-Cas9 services and products covering from sgRNA design through transfection and selection to final product verification and sequencing. CRISPR-Cas9 is a novel genome-engineering technology in recent years. Comparing with previous TALEN and ZFN technologies, CRISPR-Cas9 shows several advantages for genome editing, including simple-to-design, easy-to-use and multiplexing (capable of editing multiple genes simultaneously). The CRISPR system consists of two components, RNA fragment and Cas9 protein. Guide RNA with specific sequences is used to define the genomic target and assure the precise of this technology, while Cas9 protein cuts the genomes at defined site just like the god hand. Then double strand break (DSB) of genomic DNA occurs. Subsequently, two repair systems, NHEJ and HDR, begin to fix genome. NHEJ is an error-prone repair pathway, which may cause insertion or deletion (indel) mutations. Therefore, NHEJ can be applied for generating knockouts when indels occur within a coding exon and lead to frameshift mutations. However, HDR is a high-fidelity repair pathway, which correct the break in the presence of templates. The repair templates can either be in the form of double-stranded DNA or single-stranded DNA oligonucleotides, which target constructs with homology arms flanking the insertion sequence.

Figure 1: CRISPR-Cas9 System

CRISPR/Cas9 PlatformCB offers various genome-editing services, including but not limited to:

Reference:

  1. Lu., X. J., et al. (2015) CRISPR-Cas9: a new and promising player in gene therapy. J Med Genet 52, 289-296
  2. Ran, F. A., et al. (2013) Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8, 2281-2308

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