Genome Editing in Model Plants

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Genome Editing in Model Plants    

CRISPR/Cas9 PlatformCB utilizes genome editing technology to provide gene knockout and base editing services for customers. Various species are available, including but not limited to Arabidopsis, tobacco, B. distachyum and medicago. As a leading-edge platform around the world, we have experienced scientists, skilled staff and remarkable technology system. CRISPR/Cas9 PlatformCB will assist you with reliable and professional services of plant genome editing.

Model plant is a species that has been widely studied. With its characteristics like dwarf phenotype, short life cycle, fertility and small genome, model plant plays a crucial role in plant genomics research. Model plant is highly homologous with other complex plants in molecular level. Therefore, genome research of model plant establishes a foundation to reveal the gene function of higher plant.

Services

  • Gene knockout

Our comprehensive platform provides indels mutation, large fragment deletion and multiplex gene knockout services to meet different research purposes. To improve the mutation frequency, we respectively establish binary vector system for dicots and unary vector system for monocots. On this basis, the editing efficiency of common model plants is usually as high as 60%.

Gene Knockout ServiceFig 1. Gene Knockout Service

  • Base editing

CRISPR/Cas9 PlatformCB utilizes base editor to introduce single nucleotide change at target loci. We offer two different base editor systems named cytosine base editor (CBE) and adenine base editor (ABE). CBE achieves efficient C to T substitution, while ABE mediates the conversion of A to G in genomic DNA. Base editing systems are more efficient than HDR-mediated base pair substitution, and produce fewer undesirable mutations in the target locus. Therefore, it will be a valuable tool in genetic research of various plants.

Base Editing ServiceFig 2. Base Editing Service

Typical species

  • Arabidopsis thaliana

Arabidopsis (Arabidopsis thaliana) is the most widely used model organism in the plant field. CRISPR/Cas9, plays an important role in function verification of candidate genes and construction of mutant. Plant biologists utilize specific mutants to reveal molecular mechanisms that fight pathogens, pests and virus in Arabidopsis. CRISPR/Cas9 PlatformCB has established a mature editing system for Arabidopsis with high efficiency and short experimental period.

  • Nicotiana tabacum

Tobacco (Nicotiana tabacum) is a rich source of the alkaloid nicotine and has a long history of use as herbal medicine and social stimulant. Researchers increase yield and quality of tobacco by down-regulating the expression of negative regulators in the past. Currently, CRISPR/Cas9 provides a high accuracy breeding technique to turn tobacco leaves into biofactories of health-promoting substances such as anti-ageing or anti-inflammatory agents, as well as medicines such as vaccines or antibodies. With superior expertise and years of experience in the research of genome editing, CRISPR/Cas9 PlatformCB devotes to offering high quality service for customers.

For further information, please feel free to contact us at or 1-631-626-9181.

Reference

  1. Chen, K. L, et. al. (2019) CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. Annual Review of Plant Biology, 70:28.1–28.31.

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