SpCas9 Nickase (D10A)
Cat.No.
CCP-004
Description
D10A mutant of Cas9 introduces single-stranded DNA breaks. When used with two offset sgRNAs, creates targeted DSBs with reduced off-target effects.
Activity Assay
Nickase activity verified
Applications
High-specificity genome editing via paired nicking strategy
Buffer Composition
20 mM HEPES (pH 7.5), 150 mM KCl, 1 mM DTT, 10% glycerol
Concentration
~10 mg/mL
Endotoxin Level
<5 EU/mg
Expression System
E. coli
Molecular Weight
~160 kDa
Purity
≥95%
Shipping
Dry ice
Source Species
S. pyogenes
Storage
–80 °C
Synonyms
SpCas9 Nickase
For research use only. Not intended for any clinical use.
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