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CRISPR is the most versatile technology for genome editing and is promoting the rapid development of biomedical technology in an unprecedented way. Efficient nucleic acid delivery is a critical step in the success of genome editing experiments. Therefore, scientists need to choose the most suitable carrier according to purpose of the experiment.
In vivo applications (i.e. as a gene therapy vector) have higher requirements for vectors. For example, a vector should be required to cause a minimal immune response, greater stability, and have the ability to target a particular tissue or cell type more accurately.
The genetic material that the viral vector can deliver is usually limited to its genome size. Non-viral vectors can carry larger gene fragments.
For hard-to-transfect cells (e.g. primary cells, neurons, stem cells, etc.), it’s better to choose a vector/virus that is easily transfected.
Certain vectors provide stable long-term transgene expression, while others can only be expressed in the short term. For example, many viral vectors can be integrated into the host's genome to establish permanent gene expression in target cells.
CRISPR/Cas9 PlatformCB, with many years of experience in gene editing, is committed to providing professional and efficient products and services to customers using CRISPR system. To meet your needs, we offer a variety of vectors and viruses for the expression of sgRNA, Cas9 and variants thereof, using different vector types and different promoters to adapt Cas9 and gRNA expression to the desired cell type and/or desired the level of expression. At the same time, we offer a range of resistance screening markers (phalloidin, blasticidin, etc.), fusion marker proteins (fluorescent markers, etc.).
In addition, if you can't find the CRISPR related products you are interested in, check out our customized services. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you want to know more, please feel free to contact us.