CRISPR/Cas13 RNA Editing Services

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CRISPR/Cas13 RNA Editing Services    

Cas13 is a novel CRISPR effector protein that targets single-stranded RNA. This represents Cas13 as a potentially safe alternative to Cas9, as it induces a loss of functional phenotype without genomic changes.

Unlike most CRISPR systems that target dsDNA, Cas13 has two advanced eukaryotic and prokaryotic nucleotide-binding (HEPN) domains in tandem single-stranded RNA (ssRNA). And Cas13 has two different RNA nuclease activities:

  • RNA-activated ssRNA degradation activity
  • CRISPR RNA mature unique ribonuclease activity.

CRISPR-Cas13 targets ssRNA with its crRNA, and the twin HEPN nuclease domains cleaves the sequence non-specifically after the first crRNA guided cleavage at the binding site, leaving blunt ends. Figure 1: CRISPR-Cas13 targets ssRNA with its crRNA, and the twin HEPN nuclease domains cleaves the sequence non-specifically after the first crRNA guided cleavage at the binding site, leaving blunt ends. (Fernando Perez Rojo. 2018)

CRISPR/Cas9 PlatformCB provides customized CRISPR/Cas13 related services to support a wide range of genomic engineering applications. We can help you flexibly apply the CRISPR/Cas13 system for RNA group editing, RNA imaging in living cells, human disease gene therapy research, and the development of more potential applications for Cas13 system. With the help of an experienced team of experts and a professional technology platform, we provide customers with high-quality crRNA design and synthesis, CRISPR/Cas13 vector packaging, and dCas13 fusion protein production systems.

Our CRISPR/Cas13 genome editing services include but are not limited to

  • CRISPRCas13 crRNA design and synthesis

Based on the gene ID you provided, we will design and synthesize at least three crRNA sequences to improve the stability of crRNA in target cells and further improve target editing performance.

➢ Provide non-targeted negative control crRNA in human, mouse and rat genomes (optional)

  • CRISPR/Cas13 vector construction

➢ We provide a variety of vector designs, including crRNA only, crRNA-Cas13.
➢ TET-induced or stably expressed CRISPR/Cas13 construct.
➢ Various vectors, including non-viral and viral vectors (Lentivirus, Adenovirus, AVV).
➢ Viral vectors have the ability to transfect a variety of cells, including cells that are difficult to transfect, such as stem cells, primary cells, suspension cells, etc.

  • Cas13 mutant fusion protein construction

➢ Non-catalytically active PspCas13b is fused to the ADAR2 deaminase domain and can be used for A to I (G) replacement of reporter genes (REPAIR).

CRISPR/Cas9 PlatformCB provides the most effective and reliable services to best meet your needs and promote the development of biomedical and genetic engineering technologies.

Our characteristics and advantages

  • Have extensive CRISPR system expertise and application experience
  • High-efficiency crRNA design strategy, each gene provides three different crRNA to ensure the most efficient editing efficiency
  • Unique technology platform guarantees shorter turnaround time and lower price
  • Deliver final report detailing project process and quality monitoring data
  • Safe and fast delivery

CRISPR/Cas9 PlatformCB provides comprehensive services in the entire value chain of CRISPR system applications and technology development. Through a consulting, flexible, integrated and interdisciplinary approach, we provide advice, answers, and services to promote your project research. If you have any questions and requirements, please feel free to contact us.

Reference

  1. Fernando Perez Rojo. et al. CRISPR-Cas systems: ushering in the new genome editing era. Bioengineered. 2018; 9(1):214–221.

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