Transgene-Free Genome Editing in Plants    

Specializing in genome editing technology, CRISPR/Cas9 Platform^CB provides a comprehensive service to establish genome-edited plants with specific genome engineering modifications at your target gene/locus of interest. We also assist you to generate commercially viable target gene-edited crops, which are not limited by long and costly regulatory evaluation processes as well as by public concerns. Our mission is to produce transgene-free, regulatory free, and sustainable genome edited plants for global customers.

Genome Editing for targeted gene improvement is widely used in the field of plant science for basic research as well as for specific improvement of desired traits in commercial crops. However, a major challenge in using CRISPR gene-editing technology for agricultural applications is that the target gene-edited crop plants need to be transgene free to maintain trait stability and to gain regulatory approval for commercial production. Several strategies are employed to generate CRISPR-edited plants that do not contain CRISPR constructs and other transgenes, such as:

• Elimination of CRISPR transgenes by genetic segregation
• DNA-free genome editing system using ribonucleoprotein (RNP) or RNA
• Fluorescence marker-assisted transgene elimination
• Drug-induced elimination of transgenes
• Plant transformation and regeneration without selection

Figure 1. Strategies of transgene-free genome editing in plant

CRISPR/Cas9 Platform^CB offers transgene-free genome editing service for rice, tomato and Arabidopsis. Non-transgenic edited plants are generally obtained through genetic segregation by T0 plants selfing and crossing. Subsequently, a PCR-based genotyping is applied to identify the DNA-free T1 plants. Other strategies such as RNP/RNA based methods and marker-assisted transgene elimination are also available in CRISPR/Cas9 Platform^CB to meet specific requirements.

Workflow of transgene-free genome editing

• Project design
• Selection of targets
• Construction of CRISPR vector or preparation of RNP
• Plant transformation and regeneration with/without selection
• Mutation identification in T0 plants
• Screening of transgene-free edited plants in T1 generation

Advantages

• Advanced CRISPR/Cas9 technology platform
• Short experimental period
• Years of experience in plant genome editing
• Fully customized service to meet specific requirements

With excellent experts and years of experience in plant genome editing, we provide global customers with one-stop services from project design to transgene-free plants screening. Our plant genome editing services are proudly featured with high quality and efficiency. For further information, please feel free to contact us.