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Creative Biogene CRISPR/Cas9 Platform provides a HIEF™ site-specific gene knock-in service for customers. Based on our CRISPR/Cas9 platform, we developed a HIRF™ site-specific knock-in technology, with which single-copy target gene can be integrated into safe harbor locus (like Rosa26 locus). This technology shows a very high efficiency with or without clonal selection when integrating a single copy. You can expect that a high-yield cell line for bioproduction be received from Creative Biogene.
Integrating exogenous gene in cells (like CHO cells) is widely applied for research and industry, such as recombinant protein production and antibody production. There are three popular methods for gene integration, random insertion, knock-in by nucleases and site specific knock-in. Random insertion is uncertain in gene copies, insertion site and protein yield, and always is not reproductive. A single copy gene can be inserted into specific loci by nucleases, but always with low efficiency and long time. Comparing with the other methods, our HIEF™ site-specific knock-in method is easier and more efficient. HIEF™ site-specific knock-in method is based on recombination, so it is precise insertion and without off-target effects. When Donor plasmids is transfected into cells, recombination will occur under the control of specific enzyme.
Figure 1. HIEF™ system
CRISPR/Cas9 PlatformCB offers a site-specific knock-in service for various cell lines, especially CHO-K1 cells. With years of experiences, we have succeeded in integrating exogenous genes (such as coding gene, antibody cassette) into defined locus in CHO-K1 cells. The final cell lines show stable and uniform gene expression for long time, which are suitable for manufacturing. We are willing to assist you in high-yield cell line development with professional services.