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CRISPR/Cas9 is a powerful technology. Due to its high precision, simplicity, and flexibility, CRISPR/Cas9 has quickly become one of the most popular gene editing technologies and has completely changed the field of specific gene sequences editing. The CRISPR/Cas9 system consists of two major components: Cas9 nuclease and sequence-specific primer RNA (guide RNA, gRNA). They can form very stable ribonucleoprotein (RNP) complexes. Once a particular DNA region is matched by a gRNA carrying Cas9, the target DNA is cleaved into a double-strand break. DNA cleavage can be repaired by non-homologous end joining at the ends or by homologous DNA directed repair, which can result in alterations (deletions, insertions or mutations) in the target locus of a particular organism, resulting in altered gene function. The Cas9 RNP complex can be introduced directly into the cell and act immediately upon entry into the cell without transcription and translation. The CRISPR/Cas9 system and its variants have been widely used for gene editing applications (knockout, knock-in, gene/down, genome purification and visualization) by matching gRNA sequences to different functional domains.
As a leading genome editing biotechnology company, CRISPR/Cas9 platformCB is committed to providing customers with a range of professional CRISPR/Cas9 services and products that have been fully recognized by customers. We offer the most comprehensive, cost-effective and quality CRISPR gene editing products for basic research, pharmaceutical research, and pre-clinical research to ensure your genetic editing project goes smoothly. Our products range from sgRNA synthesis, transfection to validation, as well as knockout mice. In addition, all products have the best performance in both research and industry. In addition to products for gene editing, we still offer pre-formed knockout cell lines that can be used directly for research or production. We are committed to meeting your needs with high quality products and professional services.