Custom CRISPRi/CRISPRa Library Services    

Recently developed CRISPR/Cas9-based screening methods have great potential for revealing the mechanisms and treatment strategies of human diseases, but there are still some biological studies that are not suitable for CRISPR knockout screening. CRISPRa and CRISPRi screening opens up many new possibilities.

CRISPR/Cas9 Platform^CB provides custom libraries for any set of genes of interest, as well as non-coding RNA and regulatory domains, for genome-wide CRISPRa and CRISPRi screening in human, mouse, rat, zebrafish, C. elegans.

What is CRISPRi?

The dCas9 fusion protein targets DNA sequences through sgRNA, but the inactive dCas9 cannot catalyze DNA. Instead, a protein fused to dCas9 manipulates the transcription of the target gene.

When dCas9 is fused to the transcriptional repression domain Kruppel-associated box (KRAB), transcription is repressed, a system called CRISPR interference (CRISPRi). Compared with CRISPR cutting methods, CRISPRi is inducible, reversible and non-toxic. It can also knock down non-coding RNA.

What is CRISPRa?

When dCas9 is fused to a transcriptional activator (VPR, Sun Tag, etc.), transcription can be activated in a stable manner. This system is called CRISPR activation (CRISPRa).

Strategies for pooled genetic screens using CRISPRi or CRISPRa.

(Martin Kampmann. 2018)

What do we offer?

• Custom screening design
• Targeted gene lentivirus CRISPRi/CRISPRa library for targeted multi-species genome/transcriptome screening libraries, including human, mouse, rat, zebrafish, nematode, fruit fly, etc.
• Provides dual sgRNA library construction-contains two enhanced activations and inhibitions for each gene 5 sgRNAs designed for the promoter region of each gene for optimal inhibition or activation.
• Cell lines from pre-optimized cell series tables or cell line assessments before screening.
• Seamlessly connected with our other services and we provide genome/transcriptome nomination.
• Non-targeting sgRNA controls.

Our characteristics and advantages

• The CRISPRa system is the only method capable of genome-wide functional gain screening, which can identify endogenous genes that change phenotype when activated rather than destroyed.
• Double-sgRNA CRISPRa/CRISPRi libraries accelerate therapies for identifying new targets and analyzing biomarkers.
• Optimized sgRNA design and synthesis platform with high specificity
• Ability to target long non-coding RNA expression
• Can achieve high overexpression/interference by multiplexing sgRNAs or activator/inhibitor domains

Applications of CRISPRi/CRISPRa screening

The sgRNA library combined with dCas9 activator or repressor complex can be used for extensive gene screening.

• Assess the effects of activating or inhibiting thousands of different genes on cell growth or other screenable phenotypes.
• Identify potential therapeutic targets that are responsible for controlling growth and differentiation, regulating disease development or other biological responses.

CRISPR/Cas9 Platform^CB has solid expertise and extensive experience in genetic engineering and is committed to providing comprehensive CRISPR/Cas9 products and services for the majority of genome and proteomic researchers. We provide comprehensive solutions for the entire process of the CRISPR system for target gene editing and CRISPR library screening. Our rich experience, deep genetic engineering background, and advanced platform can guarantee to provide cost-effective high-quality services. Please feel free to contact us for further discussion.