CRISPR/Cas12a Genome Editing Services

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CRISPR/Cas12a Genome Editing Services    

The release of the Cas12a (also known as Cpf1) system in 2015 aroused widespread interest from researchers. As one of the DNA cutting proteins, CRISPR/Cas12a is complementary to Cas9 with several advantages.

  • Cas12a only uses a 20 nt DR (directed repeat) sequence preceding the crRNA (CRISPR RNA), without the need of a tracrRNA (trans-activated CRISPR RNA).
  • Cas12a extends the original spacer adjacent motif (PAM) sequence to TTTN. Therefore, the possibility of Cas12a misreading PAM on a GC-rich genome is reduced, thereby significantly improving its editing efficiency for the target gene.
  • Cpf1 cleaves target DNA 18-23 nt downstream of PAM in a staggered pattern, resulting in sticky ends. During NHEJ repair using Cas12a cleavage, the PAM site will be retained and Cas12a can be edited repeatedly (or edited for a second chance) to the same genetic target.

The extended PAM sequence, staggered cutting pattern and simplified crRNA structure make Cas12a an ideal enzyme for simple, fast, and accurate disease diagnosis.

 mechanism of CRISPR/Cas12aFigure 1: The mechanism of CRISPR/Cas12a (Zhiliang Yang. 2019)

CRISPR/Cas9 PlatformCB, a global biotechnology company, provides comprehensive CRISPR/Cas12a products and services for genomics and proteomics researchers to promote the development of basic biological research, human disease research, and drug discovery.

Our CRISPR/Cas12a genome editing services include, but are not limited

  • CRISPR/Cas12a crRNA design and synthesis

Based on the gene ID you provided, we will screen at least three PAM (TTTN) sites and synthesize your crRNA sequence using chemical modification method to improve the stability of crRNA in target cells and further improve target editing performance.

➢ All crRNAs contain a 21 base protospacer for optimal activity.
➢ Provide negative control crRNA, non-targeted in human, mouse and rat genomes (optional).

  • CRISPR/Cas12a vectors construction

➢ We offer multiple vector designs including crRNA only, crRNA-Cas12a.
➢ TET inducible or stable expressed CRISPR/Cas12a construct.
➢ Multiple vectors including non-viral and viral vectors (lentivirus, adenovirus, AVV).
➢ Viral vectors have the ability to transfect a variety of cells, including cells that are difficult to transfect, such as stem cells, primary cells, suspension cells, etc.

CRISP/Cas9 PlatformCB generates your crRNA sequence or CRISP/Cas12a vector at the best quality and best price. Our unique technology ensures fast turnaround times. In addition, we provide Cas12a (Cpf1) protein purified from E. coli and pcDNA3.1-hLbCpf1 vectors. We guarantee high-quality products and services to meet your different needs. There is no doubt, CRISP/Cas9 PlatformCB is the best partner during your research.

CRISPR/Cas12a related products

CATALOG NO. PRODUCT NAME INQUIRY
CCP-006AsCpf1 nucleaseInquiry
CCPV-013pcDNA3.1-hLbCpf1-NLSInquiry
CCPV-014pcDNA3.1-hAsCpf1Inquiry

Reference

  1. Zhiliang Yang. et al. CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica. Metabolic Engineering Communications. 2019; 10:e00112.

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