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CRISPR/Cas9 PlatformCB provides one-stop gene knockin cell line generation service, including reporter gene knockin, tag knockin and fragment knockin. This service ranges from design of gRNA, vector construction, cell transfection and selection, to single clone sequencing and cell expansion. Our talented scientists are expert in knockin cell line generation by using CRISPR system. At CRISPR/Cas9 platformCB, you can expect to achieve with satisfying services and products.
Knockin cell line, which inserted a certain fragment in a specific locus, is a useful tool for research. When a reporter gene or tag is inserted at the specific locus, this cell line can be used to study endogenous expression level of targeted gene. With the help of CRISPR/Cas9 system, it become easier to generate a custom knockin cell line. Cas9 nuclease targets to different loci of genome through sgRNA with different sequences and then the enzyme introduces double strand break. At the presence of template, DNA double strand break can be repaired via HDR pathway. By this method, promoter, tag or gene can be inserted into genome.
CRISPR/Cas9 PlatformCB offers gene knock-in services for different cell lines, such as tumor cell line and stem cell line. It is a cumbersome and time-consuming process to insert a gene in the specific site. With excellent CRISPR platform and talented scientists, we will assist you to deal with this issue and accelerate your research. We are waiting for your interesting projects.
A reporter gene is inserted in specific site and driven by endogenous promoter, which reflects the expression of targeted gene.
An inducible knockin cassette is used to generate a conditional knockin cell line. The most common used system is Cre/loxp. With the expression of Cre, the inserted gene is activated.