AsCpf1 (Cas12a) Nuclease
Cat.No.
CCP-006
Description
Recombinant Acidaminococcus sp. Cpf1 (Cas12a) protein. Recognizes TTTV PAM and introduces 5′ staggered DSBs. Requires only crRNA (no tracrRNA).
Activity Assay
Verified DNA cleavage activity
Applications
Precision genome editing and staggered DNA cleavage
Buffer Composition
20 mM HEPES (pH 7.5), 150 mM KCl, 1 mM DTT, 10% glycerol
Concentration
~10 mg/mL
Endotoxin Level
<5 EU/mg
Expression System
E. coli
Molecular Weight
~130 kDa
Purity
≥95%
Shipping
Dry ice
Source Species
Acidaminococcus sp.
Storage
–80 °C
Synonyms
AsCpf1, Cas12a
For research use only. Not intended for any clinical use.
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