SpCas9 Nuclease
Cat.No.
CCP-001
Description
Recombinant Streptococcus pyogenes Cas9 endonuclease, expressed in E. coli. Recognizes NGG PAM sequence and induces double-strand breaks (DSBs) 3 bp upstream of PAM site.
Activity Assay
in vitro nuclease activity validated
Applications
Genome editing for knockouts, knock-ins, and point mutations in mammalian cells
Buffer Composition
20 mM HEPES (pH 7.5), 150 mM KCl, 1 mM DTT, 10% glycerol
Concentration
~10 mg/mL
Endotoxin Level
<5 EU/mg
Expression System
E. coli
Molecular Weight
~160 kDa
Purity
≥95% (SEC-HPLC, SDS-PAGE)
Shipping
Dry ice
Source Species
S. pyogenes
Storage
–80 °C
Synonyms
SpCas9, Cas9
For research use only. Not intended for any clinical use.
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