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CRISPR is revolutionizing the field of genetic editing. By providing scientists with powerful tools to change any gene in any cells in a highly targeted manner without introducing foreign DNA, it has the potential to alter the process of biomedicine research and drug discovery. Compared with traditional methods, CRISPR is easy to implement and performing balletic genetic modification more efficiently. Using CRISPR/Cas9 technology, we can disable target genes by introducing frameshift mutations or removing large fragments of the gene.
The NHEJ repair pathway in the exon results in a smaller insertion or deletion, which in turn destroys the gene at the endogenous level. This is a well-established method that results in the inactivation of target genes in clonal cell lines.
DNA fragment deletion
Another method of disabling gene function is particularly useful when small insertions or deletions resulting from frameshifts are often insufficient to prevent function.
CRISPR/Cas9 PlatformCB now offers a CRISPR/Cas9 knockout cell line that combines two guide RNAs to achieve larger gene sequence excision, which ensures stable target gene disruption. Our team of professional scientists, who use CRISPR for gene editing, from the design of CRISPR gRNA constructs to the isolation and culture of transfected and single-cell clones, to cell line knockout validation.
Advantages of the knockout cell line of the CRISPR/Cas9 PlatformCB:
In addition, if you can't find the gene you're interested in, or the cell line of interest, check out our custom cell line services. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research.