CRISPR/Cas9 PlatformCB utilizes CRISPR/Cas9 technology to generate custom genome-editing animal models for customers. Various species are available, including mouse, rat, zebrafish, C.elegans. CRISPR/Cas9 PlatformCB brings customer a wide range of genetic alterations service, including knockout, point mutations, short insertions, gene knock-in and conditional knock-in. In terms of experienced scientists, skilled staff and excellent platform, we provide one-stop custom animal models generation service with high success ratio.
Genome engineering models are important tools, which are widely used in life science research, such as gene function research, cancer research and drug discovery. Eukaryotic genomes contain billions of DNA bases and are difficult to manipulate. To overcome this barrier, a series of programmable nuclease-based genome editing technologies have been developed. The most rapidly developing technology is the class of RNA-guided endonucleases known as Cas9 from the CRISPR system. Cas9 can be easily targeted to virtually any genomic location of choice by guide RNA. The ease of Cas9 targeting, high efficiency and the possibility for highly multiplexed modification broaden the application of CRISPR/Cas9 system from basic research to biotechnology and medicine. In term of the development of CRISPR/Cas9 system, the generation of genome engineering models becomes easy, fast and low-cost.
Gene Knockout animal models
Gene knockout animal models are important for studying gene function and diseases research. Conventional knockout models where gene expression is disrupted at germline level are widely used as research tools. However, deletion of some genes would cause terrible problems, such as embryonic lethal, undesired phenotypes and compensatory. To better study these gene, conditional knockout models are developed. Conditional knockout models can limit the disruption of gene expression to a particular tissue or a specific developmental stage of the animal.
Knock-in animal models
Conventional knock-in animal models are animals where a DNA sequence of interest is inserted to an endogenous locus. In term of different research purposes, conditional knockin models are developed. Conditional knockin models allow the gene insertion of interest to be activated in specific cells/tissues or temporally at specific stages.
At CRISPR/Cas9 PlatformCB, we offer custom genome engineering models to meet different needs. Our scientists are expert in CRISPR/Cas9 system and animal models generation.