Cas9 Virus Particle    

For permanent expression, sgRNA and Cas9 can be cloned into a lentiviral vector, packaged into viral particles, and transduced into target cells. Since both sgRNA and Cas9 are stably integrated into the host cell genome, they are passed on to the progeny cells. In 2014, studies (Wang et al., Science; Shalem et al., Science) showed that a library of CRISPR/Cas9 lentiviral constructs can be used to identify genes essential for mammalian cells. Moreover, CRISPR/Cas9 virus particles also can be used to help scientists create stable knockout/knock-in cell lines.

CRISPR/Cas9 Platform^CB, with years of experience in gene editing research, provides our customers with efficient Cas9 virus particles, providing an effective method to drive high levels of Cas9 nuclease expression in various cell types.

Virus we offered

• Adenovirus
• Lentivirus
• With resistance screening markers (puromycin or blasticidin)

The advantage of the CRISPR/Cas9 Platform^CB virus construct is

• A human codon-optimized version of Cas9 with a nuclear localization signal (NLS) to facilitate efficient delivery into the nucleus.
• It can be used to transduce a variety of cells and is capable of expressing Cas9 protein at high levels even in hard-to-transfect cells.
• Effectively integrates Cas9 into the genome of dividing and non-dividing cells for CRISPR genome editing.
• Enable you to create stable Cas9 expression cell pools or isolate stable Cas9 expression clones.
• Persistent expression of Cas9, reducing off-target effects.

Applications：

• Whole genome screening of CRISPR libraries
• Mammal/cell genome editing
• Functional genomics
• Drug screening and target validation
• Gene therapies

In addition, if you can't find the CRISPR related products you are interested in, check out our customized services. There is no doubt that CRISPR/Cas9 Platform^CB will be your best partner to support your research. If you want to know more, please feel free to contact us.