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For permanent expression, sgRNA and Cas9 can be cloned into a lentiviral vector, packaged into viral particles, and transduced into target cells. Since both sgRNA and Cas9 are stably integrated into the host cell genome, they are passed on to the progeny cells. In 2014, studies (Wang et al., Science; Shalem et al., Science) showed that a library of CRISPR/Cas9 lentiviral constructs can be used to identify genes essential for mammalian cells. Moreover, CRISPR/Cas9 virus particles also can be used to help scientists create stable knockout/knock-in cell lines.
CRISPR/Cas9 PlatformCB, with years of experience in gene editing research, provides our customers with efficient Cas9 virus particles, providing an effective method to drive high levels of Cas9 nuclease expression in various cell types.
Virus we offered
The advantage of the CRISPR/Cas9 PlatformCB virus construct is
In addition, if you can't find the CRISPR related products you are interested in, check out our customized services. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you want to know more, please feel free to contact us.