Conditional Knockout Cell Line Services

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Conditional Knockout Cell Line Services    

Deletion of target proteins in cells and observation of their phenotype is one of the most common methods for characterizing target protein function. However, if the loss of the target gene affects cell survival, then the acquisition of conditional knockout cells is important. Conditional knockout cell lines provide better control of gene expression and provide a more physiologically relevant model for studying the role of target genes and drug discovery. Recently, with the development of the CRISPR/Cas9 gene editing technology, it has become possible to use this technique to generate conditional knockout cell lines.

CRISPR/Cas9 PlatformCB has deep CRISPR/Cas cell line generation knowledge, extensive experience in experimental operation and data processing. Based on our platform, we are able to provide a comprehensive conditional knockout cell line generation service according to your requirements. We can design a highly optimized and efficient gene editing strategy using cell lines that are perfectly suited for your research purposes. We guarantee three homozygous (or heterozygous) clones in which the modified line has the correct genetic mutation and three vials of each clone.

Workflow of CRISPR/Cas9 conditional knockout cell line service

Although we typically use HEK239T, Hela, HepG2, U87 for CRISPR-mediated gene editing, other cell lines are still available. We provide custom cell lines according to your requirements, our scientists have extensive experience with >200 distinct cell lines and are very familiar with their culture conditions and genetic backgrounds.

Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

Why choose CRISPR/Cas9 PlatformCB?

  • Novel CRISPR strategy for higher efficiency and success rate (>98%)
  • Optional conditional knockout method
    • Combination of tetracycline-inducible Cas9 nuclease and gRNA that targets an early exon in the gene of interest.
    • Combination of auxin-inducible degron (AID) tag and CRISPR/Cas9.
  • Multiple cell lines: cancer cells, stem cells, difficult to transfect cells, most mammalian species
  • Rich experience: More than thousands of genetic editing project experience
  • Custom deliverables: homozygous/hybrid clones
  • Shorter turnaround time, lower price
  • Milestone update/report and final report

Application

  • Study the biological functions of key genes/proteins
  • Disease Modeling for Immuno-oncology, pharmacogenomics research
  • Drug discovery and drug efficacy and toxicity screening; drug combination studies
  • Antibody verification

As a professional gene editing company, CRISPR/Cas9 PlatformCB is specializing in building efficient systems and procedures to meet the needs and timelines of clients working in the CRISPR/Cas9 gene editing. We have successfully obtained thousands of CRISPR/Cas9 cell lines in >200 different cell models, including easy-to-transfer cell lines and difficult-to-transfect cell lines. Tell us your project needs, we will offer you a one-stop-shop CRISPR/Cas9 gene editing service from strategy design to final stable cell line. We guarantee our clients the most reliable and efficient research services to best match your research goals, and faster turnaround time, lower price. If you have any project need CRISPR/Cas9 cell lines, don’t hesitate to contact us.

For research use only. Not intended for any clinical use.

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