CDK5 Gene Editing

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CDK5 Gene Editing    

CDK5 (cyclin-dependent kinase 5) is a member of the cyclin-dependent kinase (CDK) family and has a basic structure and a highly conserved sequence in which the catalytic site of ATP binding is sandwiched between the apical and temporal ends. Unlike other CDKs, CDK5 is normally activated by binding to the non-cyclin CDK5 activators CDK5R1 (P35) and Cdk5R2 (P39) or their respective truncated p25 and p29. CDK5 activators are predominant in neurons, which makes CDK5 a neuron-specific CDK. CDK5-P35 plays a key role in brain development and physiological synaptic activity. In the diseased brain, tau is hyperphosphorylated by CDK5-P25, but the mechanism is still not fully understood. CDK5 activity is dysregulated in several neurological diseases leading, such as Alz Hermoside disease, Amyotrophic Lateral Sclerosis, Parkinson's disease, and Huntington's disease.

The regulatory mechanism of Cdk5 in neurons Figure 1: The regulatory mechanism of Cdk5 in neurons (Wan Liu. 2017)

CDK5 is involved in invasive cancer by reducing the activity of the actin regulatory protein calmodulin-binding protein and involved in cell cycle regulation through CCNI2, a novel CDK5 activator. More and more studies have shown that CDK5 is involved in other biological processes besides neural activity, such as gene expression, cell migration, apoptosis, myogenesis, etc.; and pathological processes including cancer, aging, diabetes, immune dysfunction, and inflammation.

CDK5 Gene Editing Service

CRISPR/Cas9 PlatformCB, one of the global leading biotechnological companies, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to support your genetic research. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we help you effectively control target genes knockout/knockin/point mutation in cells or animals via CRISPR/Cas9 technology.

  • CDK5 Gene Editing Cell Line Generation

Our professional scientists have successfully implemented CDK5 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. To support your projects, we will offer you full-length custom CDK5 gene editing service from strategy design to final stable cells. Our CDK5 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of CDK5 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

  • Alternative cell lines

➢ Blood Lineage Cells (RAW264.7, HMC1.2, K562, U937 etc.)
➢ Cancer Cell Lines (HEK293, HEK293T, Hela, MCF7, Neuro2a, HepG2, U87 etc.)
➢ Stem Cells (iPSC)
➢ Other Cell Lines (NIH3T3, MCF10, HEME, SW10 etc.)

  • CDK5 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your needs, we provide a one-stop-shop CDK5 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our CDK5 gene editing animal model generation services include:

➢ CDK5 gene conventional knockout animals
➢ CDK5 gene conditional knockout animals
➢ CDK5 point mutation animals
➢ CDK5 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services to accelerate the achievement of your research goals. We provide you with one-stop CUSTOM service to meet your special requirements with excellent quality management and quality assurance capacity. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you have any project need CRISPR system, please feel free to contact us.

Related Products at CRISPR/Cas9 PlatformCB

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References

  1. Chengcheng Liu. et al. Cyclin I-like (CCNI2) is a cyclin-dependent kinase 5 (CDK5) activator and is involved in cell cycle regulation. Scientific Reports. 2017; 7:40979.
  2. Kavita Shah. et al. Cdk5 activity in the brain – multiple paths of regulation. Journal of Cell Science. 2014; 127:2391-2400.
  3. Wan Liu. et al. Cdk5 links with DNA damage response and cancer. Molecular Cancer. 2017; 16:60.
  4. Taeko Kimura. et al. Physiological and pathological phosphorylation of tau by Cdk5. Front. Mol. Neurosci. 2014; 7:65.
  5. Zelda H. Cheung and Nancy Y. Cdk5: a multifaceted kinase in neurodegenerative diseases. Trends in Cell Biology. 2012; 22(3):169-175.
For research use only. Not intended for any clinical use.

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