CRISPR Rat for Toxicological Research    

Toxicological data are essential for any drug and for the study of drugs to understand the effects on biological systems. The severity of drug toxicity depends largely on physiological, disease, and genetic factors. In recent years, studies have found that polymorphisms in human genes alter drug metabolism and the severity of toxicity. Thus, the use of genetically engineered rodent models allows exploration of the effects of polymorphisms on drug metabolism and toxicity.

The rat is the standard experimental animal for toxicology research, and CRISPR/Cas9 gene editing has revolutionized the generation of genetically engineered rat models, providing options to accelerate and simplify the pathway for generating many different types of mutations. In many cases, CRISPR/Cas9 gene editing is a relatively simple and cost-effective alternative to gene targeting of embryonic stem (ES) cells. As such, it has rapidly become one of the leading technologies used for animal model development. And because of the ability to mount precise gene mutations, it is used to generate a variety of models valuable for basic and translational research.

Fig. 1 Immunohistochemical staining of PCNA in the liver of male and female gpt delta rats treated with MEG for 13 weeks. (Jin M, et al., 2013)

Solution

CRISPR/Cas9 Platform^CB develops rat models by adding functional genes, altering gene products, deleting genes, inserting reporter genes into regulatory sequences, replacing or repairing genes, and altering gene expression according to the needs of your project using molecular genetic techniques. We can manipulate the rat genome in a rapid and cost-effective manner and systematically generate gene editing rat models. These rat models can transform your preclinical validation process, assess potential drug response and resistance mechanisms in vivo, and ultimately guide you in the development of safer, more effective drugs.

What Can We Help You in Toxicological Research?

• Examining the role of individual genes in various toxicological outcomes
• Assessing therapeutic potential and predicts toxicity of new gene targets
• Providing mutagenicity testing, genotoxicity testing, genotoxicity testing, gene mutation detection and carcinogenesis studies
• Screening for prophylactic agents
• Screening for mutagenic and oncogenic potential and characterization of mechanisms of toxic action

Advantages of Our Technology

• Large gene alterations: up to 15kb knock-in and 400kb knock-out.
• Precise: targeted insertion for accurate expression of target genes.
• Efficient: our unique fertilization process improves homology directed repair (HDR) efficiency.
• Short timeline: gene editing rats can be produced in as little as 3-4 months

Related Services

• Knockout Rat Model Service
• Knock-in Rats Model
• Point Mutation Rat Model Service

Related Products

• CRISPR/Cas9 Kits
• Cas9 Protein
• Cas9 mRNA
• Cas9 Related Plasmids
• Pre-made Cas9 Virus Particles

CRISPR/Cas9 Platform^CB is committed to using CRISPR/Cas9 technology to select, customize, create and maintain the right gene editing models for your research and help you in your in-depth studies in toxicology.