Cas9 D10A mRNA

CCR-002

This product is capped and polyadenylated mRNA encoding Cas9 D10A nickase mutant. D10A mutation inactivates one of the nuclease domains, allowing precise DNA editing using paired sgRNAs. Ideal for reducing off-target effects.

Gene editing using Cas9 nickase (paired-nick strategy)

ARCA

~4,200

Yes

• A260/280 ratio
• RNA integrity
• Mutation site confirmation
• Endotoxin detection
• RNA/DNA contamination check

IVT mRNA

Dry ice

-80°C

5′ & 3′ UTR

Codon optimized, D10A mutation