spCas9-Puro saRNA
Cat.No.
CCR-007
Description
This self-amplifying mRNA (saRNA) encodes SpCas9 along with a puromycin resistance gene. Enables strong, prolonged Cas9 expression and facilitates selection of edited cells using puromycin.
Applications
Gene editing with puromycin selection
5′Cap
ARCA
ORF Length(nt)
>10,000 (replicon)
Poly(A) Tail
Yes
Quality Control
• A260/230 absorbance
• RNA integrity
• Endotoxin test
• Selection marker expression validation
• RNA integrity
• Endotoxin test
• Selection marker expression validation
RNA Type
saRNA
Shipping
Dry ice
Storage
≤-70°C, avoid freeze-thaw
UTR Inclusion
Complete UTR structure
Modifications
Self-replicating, IRES-puromycin
For research use only. Not intended for any clinical use.
Inquiry
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