Description
This self-amplifying RNA encodes SpCas9 and EGFP, producing long-lasting and high-level expression in target cells. Useful for live tracking of Cas9 expression. Recommended with sgRNA for editing.
Applications
Gene editing with EGFP visualization
ORF Length(nt)
>10,000 (replicon)
Quality Control
• A260/230 absorbance
• Gel integrity
• Endotoxin test
• EGFP expression confirmation
Storage
≤-70°C, avoid freeze-thaw
UTR Inclusion
Complete UTR structure
Modifications
Self-replicating, EGFP linked