Description
This mRNA encodes a fusion of SpCas9 and green fluorescent protein (GFP), enabling both genome editing and real-time visualization of Cas9 expression/localization in living cells. 5′ capped and polyadenylated. Use with sgRNA.
Applications
Gene editing with fluorescent protein tracking
Quality Control
• Fluorescence confirmation
• UV absorbance
• RNA integrity
• Endotoxin detection
• Fusion junction sequencing
Modifications
Codon optimized, GFP fusion