XIAP Gene Editing

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XIAP Gene Editing    

XIAP (X-linked inhibitor of apoptosis protein), also known as inhibitor of apoptosis protein 3 (IAP3) and an inhibitor of baculovirus containing protein 4 of the IAP repeat (BIRC4), is one of the important members of the family of apoptosis inhibition. Structurally, XIAP contains three BIRs, an ubiquitin binding (UBA) domain, and a RING finger domain (Figure 1).

XIAP Gene Editing Figure 1: Structural representation of XIAP (Stefanie Galbán. 2010).

The role of XIAP as a potent anti-apoptotic protein has been attributed to its ability to directly bind to and inhibit specific caspase (Figure 2). The BIR3 domain of XIAP binds to the amino terminus of the caspase-9, preventing its dimerization and inhibiting its protease activity. XIAP-mediated inhibition of caspase-3 and -7 is the result of binding to the BIR2 groove, which also requires an amino terminal flanking residue that can compete with proteins containing the IAP binding motif (IBM). Studies have shown that the RING domain is important for heterodimerization with c-IAP1. In addition to its anti-apoptotic properties, XIAP has been implicated in a variety of intracellular signaling events, including the NF-κB, the c-Jun-N-terminal kinase, and the TGF-β, as well as involvement in maintaining intracellular copper stability.

XIAP Gene Editing Figure 2: Role of XIAP in the extrinsic and intrinsic cell death signaling pathways (Arjmand R. Mufti. 2007).

Studies have shown that XIAP disorders can lead to cancers, neurodegenerative diseases, and autoimmune diseases, and a high proportion of XIAP has great potential to act as a tumor marker. Mutations in the XIAP gene cause severe and rare inflammatory bowel disease, and loss of XIAP leads to X-linked lymphoproliferative disorders.

XIAP Gene Editing Service

As one of the global leading biotechnology company specializing in gene editing, CRISPR/Cas9 PlatformCB is dedicated to offering comprehensive CRISPR/Cas9 related services and products to a wide range of genomics researchers. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we can help you effectively control target genes expression in vivo and in vitro with CRISPR/Cas9 technology.

  • XIAP Gene Editing Cell Line Generation

Based on our platform, we have successfully implemented XIAP CRISPR/Cas9 gene edited >200 differefnt cell lines, including hard-to-transfect cells (such as primary cells). To support your projects, we provide you with full-length custom XIAP gene editing service from strategy design to final stable cells.

➢ Our XIAP gene editing cell line generation services include

✔ gRNA design and synthesis
✔ Transfect the cell lines you’re interested
✔ Select the high expression cells and sort monoclonal cell
✔ Validate the knockout/knockin/point mutation of XIAP by PCR and sequencing
✔ Produce cryogenic preserved vials of stable cells and a final report

➢ Cell lines we offered

HEK239T, Hela, HepG2, U87, Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • XIAP Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your needs, we provide a one-stop-shop XIAP CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price.

➢ Our XIAP gene editing animal model generation services include

✔ XIAP gene conventional knockout animals
✔ XIAP gene conditional knockout animals
✔ XIAP point mutation animals
✔ XIAP knockin animals

➢ Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is dedicated to providing the best gene editing services and products for academic research, biotechnology research, and drug discovery. Our team of gene editing experts provides you with customized CRISPR / Cas9 services for any specific gene to help you solve problems encountered during the research process. We guarantee that all services and products are strictly controlled. If you have any projects that require a CRISPR system, please feel free to contact us.

Related Products at CRISPR/Cas9 PlatformCB

CATALOG NO. PRODUCT NAMEINQUIRY
CLKO-0215XIAP KO Cell Lysate-HeLaInquiry
CSC-RT0026Human XIAP Knockout Cell Line-DLD-1Inquiry
CSC-RT0054Human XIAP Knockout Cell Line-HCT116Inquiry
CSC-RT0710Human XIAP Knockout Cell Line-HeLaInquiry

References

  1. Stefanie Galbán & Colin S. Duckett. XIAP AS A UBIQUITIN LIGASE IN CELLULAR SIGNALING. Cell Death Differ. 2010; 17(1):54–60.
  2. Arjmand R. Mufti. et al. XIAP: CELL DEATH REGULATION MEETS COPPER HOMEOSTASIS. Arch Biochem Biophys. 2007; 463(2):168–174.
  3. Wilkinson JC. et al. Upstream regulatory role for XIAP in receptor-mediated apoptosis. Molecular and Cellular Biology. 2004; 24(16):7003–14.
  4. Yasuyuki Suzuki. et al. X-linked Inhibitor of Apoptosis Protein (XIAP) Inhibits Caspase-3 and -7 in Distinct Modes. The Journal of Biological Chemistry. 2001; 276:27058-27063.
  5. Yihua Huang. et al. Structural Basis of Caspase Inhibition by XIAP. Differential Roles of the Linker versus the BIR Domain. Cell. 2001; 104(5):781-790.
For research use only. Not intended for any clinical use.

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