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SMO, also known as smoothened, and frizzled class receptor, is a seven-transmembrane G protein-coupled receptor, a key component of the hedgehog signaling pathway. The SMO/HH signaling pathway is one of the most important evolutionarily conserved pathways involved in embryonic development and cancer and is involved in the formation and homeostasis of various tissues and organ systems. HH induces SMO phosphorylation and cell-surface accumulation, and the glioma-associated oncogene homolog (GLI) protein is the major transcriptional effector of the HH/SMO signal.
As an intracellular multifunctional protein, SMO is a molecular target of the teratogen cyclopamine and has also been shown to bind to actin Costal-2 and play a role in the localization of the Ci complex. Studies have shown that SMO can act as a proto-oncogene, and activation of SMO mutations can lead to activation of the unregulated hedgehog pathway. SMO abnormalities are closely related to diseases such as -Jones syndrome and basal cell carcinoma. As its function, SMO is an attractive targeted-drug in cancer treatment. And SMO inhibitors provide a new framework for drug development in cancer treatment.
Figure 1: A diagram of HH/SMO signaling in mammalian cells
SMO Gene Editing Service
CRISPR/Cas9 PlatformCB is one of the leading genomic editing companies and specialized in providing a set of services for CRISPR-Cas9 genome editing. We have national-class labs, a team of professional scientists from world-level universities. Based on our platform, we can provide you CRISPR/Cas9 genomic editing services from strategy design to genetic edited cell lines or animal models to meet your specific project needs and provide experienced scientific support at every step.
CRISPR/Cas9 PlatformCB has successfully implemented gene editing of SMO in a variety of cell lines, including easy-to-transfect cell lines and hard-to-transfect cells. Our one-stop-shop services of CRISPR/Cas9 gene editing cell lines cover from sgRNA design to final cell line generation/verification, which have been well successful and recognized by our customers. Our SMO gene editing cell line generation services include:
✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of SMO by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report
Typically, we develop CRISPR-mediated gene-editing cell lines, including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.
Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.
CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models. We have obtained >300 mouse models with very high efficiency and success rate by using CRISPR/Cas9-mediated genome editing technology. We can provide you with CRISPR/Cas SMO gene-editing animal models at a reasonable cost and in a shorter time. Our SMO gene editing animal model generation services include:
➢ SMO gene conventional knockout animals
➢ SMO gene conditional knockout animals
➢ SMO point mutation animals
➢ SMO knockin animals
Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.
CRISPR/Cas9 PlatformCB is specializing in providing custom CRISPR/Cas genome engineering projects. Tell us your needs, we will provide you with a professional genetic edit strategy and design. We guarantee our clients the most reliable and efficient research services to best match your research goals.
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