PARP3 Gene Editing

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PARP3 Gene Editing    

PARP (Poly (ADP-ribose) polymerase), represents a family of proteins involved in many key cellular processes associated with genomic DNA integrity, such as DNA repair, genomic stability, and cellular stress response. The entire human family includes 17 members with very different structural and cellular functions, but these members are associated with a conserved PARP catalytic domain.

PARP3 is a DNA-dependent single (ADP-ribose) transferase that is primarily localized to the cytoplasm and nuclear spots, and originally found in EST library screening using the catalytic domain sequence of hPARP1. Expression analysis revealed that the strict regulation of PARP3 is different from the commonly expressed PARP1 and PARP2. PARP3 is involved in transcriptional silencing and DNA repair networks, including base excision repair/single strand break repair (BER/SSBR) and non-homologous end joining (NHEJ), suggesting an active role for PARP3 in maintaining genomic integrity.

PARP3 Gene Editing Service

CRISPR/Cas9 PlatformCB, one of the global leading biotechnological companies, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products for academic research, biotech research and pharmaceutical drug discovery. To support your projects, we provide you with effectively controlled target genes knockout/knockin/point mutation in cells or animals using CRISPR/Cas9 technology.

  • PARP3 Gene Editing Cell Line Generation

Our professional scientists have successfully implemented PARP3 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. Based on our platform, we will offer you full-length custom PARP3 gene editing service from strategy design to final stable cells. Our PARP3 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of PARP3 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • PARP3 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your projects’ needs, we provide a one-stop-shop PARP3 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our PARP3 gene editing animal model generation services include:

➢ PARP3 gene conventional knockout animals
➢ PARP3 gene conditional knockout animals
➢ PARP3 point mutation animals
➢ PARP3 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services to accelerate the achievement of your research goals. We provide you with one-stop custom service to meet your special requirements with excellent quality management and quality assurance capacity. If you have any questions, please feel free to contact us.

Related Products at CRISPR/Cas9 PlatformCB

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CLKO-2127PARP3 KO Cell Lysate-HelaInquiry
CSC-RT2473Human PARP3 Knockout Cell Line-HelaInquiry

References

  1. E. A. Belousova. et al. Dna is a New Target of Parp3. Scientific Reports. 2018; 8: 4176.
  2. Christian Boehler. et al. Poly(ADP-ribose) polymerase 3 (PARP3), a newcomer in cellular response to DNA damage and mitotic progression. PNAS. 2011; 108 (7) 2783-2788.
  3. Johansson M. et al. A human poly(ADP-ribose) polymerase gene family (ADPRTL): cDNA cloning of two novel poly(ADP-ribose) polymerase homologues. Genomics. 1999; 57:442–445.
  4. Vyas, S. et al. Family-wide analysis of poly(ADP-ribose) polymerase activity. Nat. Commun. 2014; 5:4426.
For research use only. Not intended for any clinical use.

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