PARP2 Gene Editing

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PARP2 Gene Editing    

PARP2 (poly (ADP-ribose) polymerase-2) belongs to the ADP-ribosyltransferase family. PARP2, PARP1 and PARP3 are the only known DNA damage-dependent PARPs that play key roles in DNA damage responses, DNA metabolism and chromatin structure. The PARP2 gene, located on chromosomes 14q11.2 (human) and 14C1 (murine), is approximately 13 kb and consists of 16 exons and intron 15, encodes different PARP2 protein isoforms. However, the biological significance of these protein variants is largely unknown.

Structurally, the 62kDa PARP2 protein contains the N-terminal region (NTR), the central WGR (Trp-Gly-Arg) domain and the C-terminal catalytic domain (CAT). The CAT domain is composed of a helical subdomain (HD) and the ADP-ribosyltransferase (ART) subdomain, which allow coupling of catalysis to DNA break detection. Studies have shown that PARP2 NTR is not required for DNA binding, but is critical for PARP2 activation on SSB.

Functionally, PARP2 is involved in maintaining genomic stability (under the conditions of replication stress, DNA repair, chromatin structure, and epigenetic modification, chromosome segregation, cell cycle regulation, telomere maintenance), proliferative signaling, glycolipid metabolism, angiogenesis, inflammation, immune regulation, and immune evasion (Figure 1). Constant discoveries of the specific biological functions of PARP2 show that PARP2 is involved in many tumorigenesis, invasion, and immune evasion processes suggesting the possibility of PARP2 as a specific target in cancer therapy. Furthermore, the emerging role of PARP2 in the regulation of the tumor suppressor TGFβ signaling cascade may provide another way to prevent tumor proliferation.

Figure 1: PARP2 executes its biological functions via PARylation (Syed O Ali. 2016)

PARP2 Gene Editing Service

CRISPR/Cas9 PlatformCB, one of the globle leading biotechnological companies, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products for academic research, biotech research and pharmaceutical drug discovery. Based on our platform, we are able to help you effectively control target genes knockout/knockin/point mutation in cells or animals via CRISPR/Cas9 technology.

  • PARP2 Gene Editing Cell Line Generation

Our professional scientists have successfully implemented PARP2 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. To support your projects, we will offer you full-length custom PARP2 gene editing service from strategy design to final stable cells. Our PARP2 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of PARP2 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • PARP2 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your projects’ needs, we provide a one-stop-shop PARP2 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our PARP2 gene editing animal model generation services include:

➢ PARP2 gene conventional knockout animals
➢ PARP2 gene conditional knockout animals
➢ PARP2 point mutation animals
➢ PARP2 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB has many years of experience in providing the best gene editing services to accelerate the achievement of your research goals. We provide you with one-stop custom service to meet your special requirements with excellent quality management and quality assurance capacity. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research.

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References

  1. Hottiger MO. Nuclear ADP-ribosylation and its role in chromatin plasticity, cell differentiation, and epigenetics. Ann Rev Biochem. 2015; 84:227-263.
  2. Syed O Ali. et al. Understanding specific functions of PARP-2: new lessons for cancer therapy. Am J Cancer Res. 2016; 6(9):1842-1863.
  3. Péter Bai and Carles Cantó. The Role of PARP-1 and PARP-2 Enzymes in Metabolic Regulation and Disease. Cell Metabolism. 2012; 16(3):290-295.
  4. Qian Chen. et al. PARP2 mediates branched poly ADP-ribosylation in response to DNA damage. Nature Communications. 2018; 9:3233.
  5. M. M. Kutuzov. et al. Role of PARP2 in DNA repair. Molecular Biology. 2014; 48(4):485-495.
For research use only. Not intended for any clinical use.

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