HDAC6 Gene Editing

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HDAC6 Gene Editing    

HDAC6 (Histone deacetylase 6) is a member of the class IIb HDAC family with two active catalytic domains and unique physiological functions (Figure 1). In addition to histone modifications, HDAC6 also targets specific substrates, including alpha-tubulin, HSP90 and cortactin, and is involved in protein trafficking and degradation, cell morphology and migration, autophagy, neurotransmitter release, and vesicles, mitochondrial transport, maturation of the glucocorticoid receptor. Importantly, emerging evidence also suggests that HDAC6 is regulated in the immune response, particularly at APC/T cell immune synaptic levels.

Figure 1: Functional domain organization of HDAC6 (C Boyault. 2007)

Mutations in the HDAC6 gene are associated with Alzheimer's disease, HDAC inhibitors act as novel therapeutic agents for aging and Alzheimer's disease. Overexpression of HDAC6 is involved in tumorigenesis and cell survival, and in addition, promotes metastasis of cancer cells; Therefore, HDAC6 can be used as a marker for prognosis. The major substrate for HDAC is alpha-tubulin, which has become a target for drug development in cancer treatment due to its major contribution to cancer cell transformation.

HDAC6 Gene Editing Service

As one of the global leading biotechnological companies, CRISPR/Cas9 PlatformCB is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products for academic research, biotech research and pharmaceutical drug discovery. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we can help you effectively control target genes deleted, inserted or point mutation in cells or animals via CRISPR/Cas9 technology.

  • HDAC6 Gene Editing Cell Line Generation

Our professional scientists have extensive experience with >200 distinct cell lines and successfully implemented HDAC6 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. Tell us your needs, we will offer you full-length custom HDAC6 gene editing service from strategy design to final stable cells. Our HDAC6 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of HDAC6 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • HDAC6 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been fully recognized by our clients. To support your projects, we provide a one-stop-shop HDAC6 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our HDAC6 gene editing animal model generation services include:

➢ HDAC6 gene conventional knockout animals
➢ HDAC6 gene conditional knockout animals
➢ HDAC6 point mutation animals
➢ HDAC6 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is specializing in building efficient systems and procedures to meet the needs and timelines of clients working in the CRISPR/Cas9 gene editing. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services and products for any specific gene to help you solve problems encountered during your research with excellent quality management and quality assurance capacity. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you have any projects needing the CRISPR system, please feel free to contact us.

Related Products at CRISPR/Cas9 PlatformCB

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References

  1. Grace I. Aldana-Masangkay and Kathleen M. Sakamoto. The Role of HDAC6 in Cancer. J Biomed Biotechnol. 2011; 2011:875824.
  2. Sri N. Batchu. et al. The therapeutic hope for HDAC6 inhibitors in malignancy and chronic disease. Clinical Science. 2016, 130(12):987-1003.
  3. C Boyault. et al. HDAC6, at the crossroads between cytoskeleton and cell signaling by acetylation and ubiquitination. Oncogene. 2007; 26:5468-5476.
  4. Dongjie Zhou. et al. Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice. Scientific Reports. 2017; 7:8131.
  5. AlexiNott. et al. HDAC Inhibitors as Novel Therapeutics in Aging and Alzheimer’s Disease. Epigenetic Regulation in the Nervous System. 2013:225-248.
  6. Claudia Simões-Pires. et al. HDAC6 as a target for neurodegenerative diseases: what makes it different from the other HDACs? Molecular Neurodegeneration. 2013; 8:7.
For research use only. Not intended for any clinical use.

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