HDAC2 Gene Editing

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HDAC2 Gene Editing    

HDAC2 (histone deacetylase 2) is a ubiquitously expressed nuclear protein belonging to class I HDACs (HDAC1, HDAC2, HDAC3 and HDAC8). HDACs act by forming large polyprotein complexes and are responsible for the deacetylation of lysine residues in the N-terminal region of core histones (H2A, H2B, H3 and H4). This protein forms a transcriptional repressor complex by binding to many different proteins, including the mammalian zinc finger transcription factor YY1. Therefore, it plays an important role in transcriptional regulation, cell cycle progression and developmental events.

HDAC1 and HDAC2 are known to act as modulators of cell cycle, angiogenesis, apoptosis, differentiation and metastasis. In contrast to its role in chromatin remodeling complexes, HDAC1 and HDAC2 inhibit the p21 gene promoter. In addition, HDAC1 and HDAC2 play important roles in promoting the activity of oncogenic DNA-binding fusion proteins. Whether HDAC1 and HDAC2 have completely independent inhibitory functions, gene targets or effects on cell function are unclear. As HDAC2 is abnormally expressed in several types of cancer, including gastric cancer, colorectal cancer, prostate cancer, and Hodgkin's lymphoma. The identification of the exact function of HDAC2 is still important for understanding the epigenetic mechanisms of human disease because the contribution of HDAC2 to tumors genesis may be independent of HDAC1.

Figure 2: Physiological processes influenced through HDAC2 via deacetylation of histones (H) and non-histones (N) (Krämer. O. H. 2009)

HDAC2 Gene Editing Service

CRISPR/Cas9 PlatformCB, one of the global leading biotechnological companies specializing in gene editing, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to support your genetic research. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we help you effectively control target genes edited (including deletion, insertion and point mutation) in cells or animals via CRISPR/Cas9 technology.

  • HDAC2 Gene Editing Cell Line Generation

We have successfully implemented HDAC2 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. Tell us your needs, we offer you full-length custom HDAC2 gene editing service from strategy design to final stable cells. Our HDAC2 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of HDAC2 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • HDAC2 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been fully recognized by our clients. To support your projects, we provide a one-stop-shop HDAC2 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our HDAC2 gene editing animal model generation services include:

➢ HDAC2 gene conventional knockout animals
➢ HDAC2 gene conditional knockout animals
➢ HDAC2 point mutation animals
➢ HDAC2 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services and products for academic research, biotech research and pharmaceutical drug discovery with excellent quality management and quality assurance capacity. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services for any specific gene to help you solve problems encountered during your research. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you have any projects needing CRISPR system, please feel free to contact us.

Related Products at CRISPR/Cas9 PlatformCB

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Reference

  1. ShaneO’Grady. et al. Liver Cancer (Hepatocellular Carcinoma). Epigenetic Cancer Therapy. 2015; 269-288.
  2. Betz R. et al. Human histone deacetylase 2, HDAC2 (Human RPD3), is localized to 6q21 by radiation hybrid mapping. Genomics. 1998; 52 (2): 245-6.
  3. Krämer, O. H. HDAC2: a critical factor in health and disease. Trends in Pharmacological Sciences. 2009; 30(12), 647-655.
  4. Priyanka Somanath. et al. CRISPR-mediated HDAC2 disruption identifies two distinct classes of target genes in human cells. PLoS One. 2017; 12(10):e0185627.
For research use only. Not intended for any clinical use.

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