HDAC1 Gene Editing

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HDAC1 Gene Editing    

HDAC1, a member of the histone deacetylase (HDACs) family, is a family of proteins based on the ability to initially identify lysine residues on its deacetylated histones, resulting in the compaction of chromatin transcriptional repression status. HDACs catalyze the deacetylation of histones and many other non-histone proteins such as tubulin and transcription factors such as TRP53 (P53), E2F1 and CREB, which in turn affect cell life processes. HDAC1 deacetylates TSHZ3 and regulates its transcriptional inhibitory activity, deacetylating 'Lys-310' in RELA, thereby inhibiting the transcriptional activity of NF-κB. Deacetylation of NR1D2 and elimination of KAT5-mediated NR1D2 transcriptional repressor activity.

HDAC1 forms a complex with BRG1, RB1 and negatively regulates CREST-mediated resting neuronal transcription. After calcium stimulation, HDAC1 is released from the complex, recruiting CREBBP and promoting transcriptional activation. Recent studies have shown that HDAC1 is a molecular switch between neuronal survival and death. Its interaction with HDRP promotes neuronal survival, while interaction with HDAC3 leads to neuronal death. And the germline deletion of HDAC1 can lead to mouse embryonic lethality before embryonic period 10.5.

HDAC1 Gene Editing Service

CRISPR/Cas9 PlatformCB is one of the leading gene editing companies and committed to offering comprehensive CRISPR/Cas9 gene editing services and products to solve the challenging problems of CRISPR technology applications and support your genetic research. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we help you effectively control target genes knockout/knockin/point mutation in cells or animals via CRISPR/Cas9 technology.

  • HDAC1 Gene Editing Cell Line Generation

Based on advanced national-level labs, a team of professional scientists, CRISPR/Cas9 PlatformCB has successfully implemented HDAC1 CRISPR/Cas9 gene edit in both easy-to-transfect cell lines and hard-to-transfect cells. Tell us your needs, we will offer you professional custom HDAC1 gene editing services from strategy design to final stable cells. Our HDAC1 gene editing cell line generation services include:

  • SgRNA design and synthesis
  • Transfect the cell line you interest
  • Select the high expression cell and sort monoclonal cell
  • Validate the knockout/knockin/point mutation of HDAC1 by PCR and sequencing
  • Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • HDAC1 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been fully recognized by our clients. According to your projects' needs, we provide a one-stop-shop HDAC1 CRISPR/Cas9 gene editing animal services and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our HDAC1 gene editing animal model generation services include:

  • HDAC1 gene conventional knockout animals
  • HDAC1 gene conditional knockout animals
  • HDAC1 point mutation animals
  • HDAC1 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services to accelerate the achievement of your research goals. Our one-stop service ranges from strategy design to final stable cell lines or F1 animals. In addition, we provide you with custom service to meet your special requirements with excellent quality management and quality assurance capacity. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you have any project need CRISPR system, don't hesitate to contact us.

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References

  1. Farah H. Bardai. et al. Histone Deacetylase-1 (HDAC1) Is a Molecular Switch between Neuronal Survival and Death. The Journal of Biological Chemistry. 2012; 287, 35444-35453.
  2. Shereen Jamaladdin. et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. PNAS. 2014; 111 (27):9840-9845.
  3. Pengpeng Ma. et al. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. PNAS. 2012; 109 (8):E481-E489.
For research use only. Not intended for any clinical use.

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