Fyn Gene Editing

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Fyn Gene Editing    

Fyn is a 59 kDa non-receptor tyrosine kinase belonging to the Src family kinase (SFK). The Fyn gene is located on chromosome 6q21 and expresses slightly different isomers. Among them, FynT is mainly expressed in thymocytes, spleen cells, and some blood lymphocyte lines. FynB is ubiquitous and has a particularly high expression level in the brain. The biological functions of Fyn are diverse (Figure 1). It participates in signal transduction pathways especially related to myelination in the nervous system under normal physiological conditions, as well as the development and activation of T lymphocytes. In cancer, Fyn promotes the development and progression of several cancer types by participating in the control of cell growth, death, morphogenesis, and cell movement.

One of the most well-known functions of Fyn kinase is the phosphorylation of SLAM (signaling lymphocyte activating molecule) in T cells, which induces signaling complexes that regulate interferon-gamma expression. FynT is an important mediator of T cell signaling and differentiation of CD4+ cells into T helper type 2 cells in rodents, which are critical for humoral immune responses. Recently, studies have shown that Fyn exists in the pathogenesis of AD, and its interaction with both Aβ and Tau renders Fyn a unique therapeutic target that addresses both of the major pathologic hallmarks of AD. Fyn also affects the blood system and binds to glycoprotein IIb-IIIa on platelets, a well-known integrin that has become a target for cardiovascular disease and stroke. In addition, increased expression and/or activation of Fyn is observed in various cancers, including melanoma, glioblastoma, squamous cell carcinoma, prostate cancer, and breast cancer. Studies have demonstrated the importance of Fyn in the resistance or susceptibility of cancer cells to certain anticancer therapies.

 Overview of the molecular and cellular effects of FynFigure 1: Overview of the molecular and cellular effects of Fyn

Fyn Gene Editing Service

As one of the leading gene editing companies, CRISPR/Cas9 PlatformCB has focused on building efficient systems and procedures to meet the needs and timelines of clients working in the CRISPR/Cas9 gene editing. Our scientists have deep gene editing knowledge and extensive experience in experimental operation and data processing. Based on our platform, CRISPR/Cas9 PlatformCB offers CRISPR/Cas gene editing services and helps you achieve specific gene editing including knockout, knockin, or point mutation in vitro and in vivo, within shorter turnaround time.

  • Fyn Gene Editing Cell Line Generation

CRISPR/Cas9 PlatformCB has successfully implemented Fyn CRISPR/Cas9 gene editing both easy-to-transfect cell lines and hard-to-transfect cells. Tell us the cell lines your project needs, we will offer you a professional one-stop-shop Fyn gene editing service from strategy design to final stable cells at a reasonable cost and in a shorter time. Our Fyn gene editing cell line generation services include:

✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of Fyn by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines, including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • Fyn Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been recognized by our clients. We provide you with custom Fyn CRISPR/Cas gene editing animals and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower cost. Our Fyn gene editing animal model generation services include:

➢ Fyn gene conventional knockout animals
➢ Fyn gene conditional knockout animals
➢ Fyn point mutation animals
➢ Fyn knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

Tell us your needs, CRISPR/Cas9 PlatformCB will provide you a professional CRISPR/Cas9 gene editing service. With our national-class labs and gene editing scientists from world-class universities, we guarantee our clients the most reliable and efficient research services to best match your research goals and protect your science investment.

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References:

  1. Haakon B. Nygaard, MD. Targeting Fyn Kinase in Alzheimer's Disease. Biol Psychiatry. 2018; 83(4): 369–376.
  2. Emil H Palacios & Arthur Weiss. Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation. Nature Oncogene. 2004;23, 7990–8000.
  3. Chan Gyu Lee. et al. Phytochemical regulation of Fyn and AMPK signaling circuitry. Archives of Pharmacal Research. 2015; 38(12): 2093–2105.
  4. Daniel Eliasa. Henrik J.Ditzelab. Fyn is an important molecule in cancer pathogenesis and drug resistance. Pharmacological Research. 2015; 100: 250-254.
For research use only. Not intended for any clinical use.

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